Skip Navigation

This Article
Right arrow Full Text Freely available
Right arrow FREE Full Text (PDF ) Freely available
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (13)
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Zetterberg, H.
Right arrow Articles by Blennow, K.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Zetterberg, H.
Right arrow Articles by Blennow, K.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Human Reproduction, Vol. 17, No. 12, 3033-3036, December 2002
© 2002 European Society of Human Reproduction and Embryology

The transcobalamin codon 259 polymorphism influences the risk of human spontaneous abortion

H. Zetterberg1,5, B. Regland2, M. Palmér1, L. Rymo1, A. Zafiropoulos3, D.A. Arvanitis3, D.A. Spandidos3 and K. Blennow1,4

1 Department of Clinical Chemistry and Transfusion Medicine, Sahlgrenska University Hospital, Göteborg University, S-413 45 Gothenburg, 2 Institute of Clinical Neuroscience, Psychiatry Section, Sahlgrenska University Hospital, Göteborg University, S-431 80 Mölndal, Sweden, 3 Department of Virology, Medical School, University of Crete, Heraklion, Crete, P.O. Box 1393, Greece and 4 Institute of Clinical Neuroscience, Department of Experimental Neuroscience, Sahlgrenska University Hospital, Göteborg University, S-431 80 Mölndal, Sweden

BACKGROUND: The remethylation cycle of methionine is folate and vitamin B12 (cobalamin) dependent and appears to be crucial for embryonic development, probably through effects on synthesis of DNA, proteins and polyamines. Transcobalamin (TC) transports vitamin B12 to the tissues. The objective of the present investigation was to explore the putative association between the major TC genetic polymorphism (Pro259Arg) and human spontaneous abortion. METHODS: The prevalence of the TC Pro259Arg polymorphism was determined in DNA samples from embryos that had been spontaneously aborted between the 6th and 20th week after conception, and adult controls using solid-phase minisequencing technique. RESULTS: The 259-Pro allele was significantly less frequent in the spontaneous abortion group than in the control group (42.2 and 57.0% respectively; P = 0.005), while the frequency of 259-Arg was significantly increased. There was a lower prevalence of 259-Pro homozygotes in the spontaneous abortion group compared with the control group (9.1 and 32.2% respectively; P < 0.001). CONCLUSIONS: The 259-Pro allele seems to have beneficial influences during embryogenesis, conceivably through its positive effect on vitamin B12 intracellular bioavailability. Our results warrant additional investigations addressing the question if vitamin B12 supplementation in addition to folic acid supplementation may prevent spontaneous abortion in women planning a pregnancy.

Key words: B12/homocysteine/polymorphism/spontaneous abortion/transcobalamin

5 To whom correspondence should be addressed. E-mail: henrik.zetterberg{at}clinchem.gu.se


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 J. Med. Genet.Home page
J.-L. Gueant, N. W Chabi, R.-M. Gueant-Rodriguez, O. M Mutchinick, R. Debard, C. Payet, X. Lu, C. Villaume, J.-P. Bronowicki, E. V Quadros, et al.
Environmental influence on the worldwide prevalence of a 776C->G variant in the transcobalamin gene (TCN2)
J. Med. Genet., June 1, 2007; 44(6): 363 - 367.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Clin. Nutr.Home page
R.-M. Gueant-Rodriguez, J.-L. Gueant, R. Debard, S. Thirion, L. X. Hong, J.-P. Bronowicki, F. Namour, N. W Chabi, A. Sanni, G. Anello, et al.
Prevalence of methylenetetrahydrofolate reductase 677T and 1298C alleles and folate status: a comparative study in Mexican, West African, and European populations
Am. J. Clinical Nutrition, March 1, 2006; 83(3): 701 - 707.
[Abstract] [Full Text] [PDF]

Home page
 Mol Hum ReprodHome page
A. Parle-McDermott, F. Pangilinan, J. L. Mills, C. C. Signore, A. M. Molloy, A. Cotter, M. Conley, C. Cox, P. N. Kirke, J. M. Scott, et al.
A polymorphism in the MTHFD1 gene increases a mother's risk of having an unexplained second trimester pregnancy loss
Mol. Hum. Reprod., July 1, 2005; 11(7): 477 - 480.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Chem.Home page
K. Meyer, A. Fredriksen, and P. M. Ueland
High-Level Multiplex Genotyping of Polymorphisms Involved in Folate or Homocysteine Metabolism by Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry
Clin. Chem., February 1, 2004; 50(2): 391 - 402.
[Abstract] [Full Text] [PDF]

Home page
 Hum ReprodHome page
H. Zetterberg, A. Zafiropoulos, D. A. Spandidos, L. Rymo, and K. Blennow
Gene-gene interaction between fetal MTHFR 677C>T and transcobalamin 776C>G polymorphisms in human spontaneous abortion
Hum. Reprod., September 1, 2003; 18(9): 1948 - 1950.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
H. Zetterberg, M. Palmer, C. Borestrom, L. Rymo, K. Blennow, J. W. Miller, and R. Green
The transcobalamin codon 259 polymorphism should be designated 776C>G, not 775G>C
Blood, May 1, 2003; 101(9): 3749 - 3750.
[Full Text] [PDF]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.