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Human Reproduction, Vol. 17, No. 12, 3190-3192, December 2002
© 2002 European Society of Human Reproduction and Embryology

Few instead of many: human follicle collection from follicular aspirates at oocyte retrieval

Pu Zhang1,3, Julius Gisli Hreinsson1, Evelyn Telfer2 and Outi Hovatta1

1 Department of Clinical Science, Division of Obstetrics and Gynaecology, Karolinska Institutet, Huddinge University Hospital, S-141 86 Stockholm, Sweden and 2 Institute of Cell and Molecular Biology, University of Edinburgh, Darwin Building, Mayfield Road, Edinburgh EH9, UK

BACKGROUND: The aim of this study was to determine if follicular aspirates obtained during oocyte retrieval for IVF were a good source of ovarian follicles for research purposes. METHODS: Follicular aspirates from 86 patients were collected and examined for the presence of follicles, and histological examination of tissue sample found was undertaken. RESULTS: Follicles were only obtained from aspirates of seven out of a total of 86 patients. From these samples a total of 14 follicles was found. The follicles were primordial, primary or secondary, 40–80 µm in diameter. Three of these recovered follicles were cultured and all degenerated within 2 days. In all aspirates some groups of granulosa cells that did not contain follicles or oocytes were found, as was vaginal epithelium that was also identified and verified by histology. CONCLUSIONS: Follicular aspirates are not a useful source of human follicles. Some structures found in the aspirates may be erroneously identified as follicles.

Key words: follicular fluid/human/IVF/oocyte/ovarian follicle

3 To whom correspondence should be addressed. E-mail: Pu.Zhang{at}klinvet.ki.se


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