Skip Navigation

This Article
Right arrow Full Text Freely available
Right arrow FREE Full Text (PDF ) Freely available
Right arrow Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (7)
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Sbracia, M.
Right arrow Articles by Aragona, C.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Sbracia, M.
Right arrow Articles by Aragona, C.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Human Reproduction, Vol. 17, No. 2, 320-324, February 2002
© 2002 European Society of Human Reproduction and Embryology

Preferential location of sex chromosomes, their aneuploidy in human sperm, and their role in determining sex chromosome aneuploidy in embryos after ICSI

M. Sbracia1,5, M. Baldi2, D. Cao3, A. Sandrelli2, A. Chiandetti3, R. Poverini1 and C. Aragona4

1 Centro di Endocrinologia e Medicina della Riproduzione, 2 Consultorio di Genetica, 3 Università `Tor Vergata' and 4 II Clinica Ostetrica e Ginecologica, Università `La Sapienza' Rome, Italy

BACKGROUND: In babies born after ICSI procedures, an increase of de-novo sex chromosome abnormalities has been observed. Several hypotheses have been proposed to explain these findings: an increased rate of sex chromosome aneuploidy in sperm of oligozoospermic men, or a preferential location of the sex chromosomes in the sub-acrosomal region of the sperm nucleus which leads to a reduced DNA decondensation of this region. In order to investigate which theory may be more reliable, we studied the localization of sex chromosomes and their aneuploidy rate in sperm in men undergoing ICSI. METHODS: Using fluorescent in-situ hybridization we studied sex chromosome localization and the aneuploidy rate for sex chromosomes and chromosome 18 in 20 oligospermic men undergoing ICSI and in 10 controls. RESULTS: In 40.94 and 52.92% of cases, the X and Y chromosomes respectively were localized in the sub-acrosomal region of the sperm nucleus compared with only 14.29% of cases of chromosome 18 (P < 0.001). An increase of sex chromosome aneuploidy in sperm of oligospermic men was observed; 2.91 versus 0.69% of controls (P < 0.001). CONCLUSIONS: Sex chromosomes are localized preferentially in the sub-acrosomal region of sperm and sex chromosome aneuploidy rate in the sperm of oligozoospermic men is higher than in controls.

Key words: aneuploidy/FISH/ICSI/sex chromosomes

5 To whom correspondence should be addressed at: Center for Endocrinology and Reproductive Medicine, Via Carlo Porta 10, 00153, Rome, Italy. E-mail: marcandrea{at}hotmail.com

Submitted on April 23, 2001


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Hum ReprodHome page
K.A. Finch, K.G.L. Fonseka, A. Abogrein, D. Ioannou, A.H. Handyside, A.R. Thornhill, N. Hickson, and D.K. Griffin
Nuclear organization in human sperm: preliminary evidence for altered sex chromosome centromere position in infertile males
Hum. Reprod., June 1, 2008; 23(6): 1263 - 1270.
[Abstract] [Full Text] [PDF]

Home page
 Hum ReprodHome page
D.K. Griffin, P. Hyland, H.G. Tempest, and S.T. Homa
Safety issues in assisted reproduction technology: Should men undergoing ICSI be screened for chromosome abnormalities in their sperm?
Hum. Reprod., February 1, 2003; 18(2): 229 - 235.
[Abstract] [Full Text] [PDF]

Home page
 Hum ReprodHome page
G. Tachdjian, N. Frydman, N. Morichon-Delvallez, A. L. Du, R. Fanchin, M. Vekemans, and R. Frydman
Reproductive genetic counselling in non-mosaic 47,XXY patients: implications for preimplantation or prenatal diagnosis: Case report and review
Hum. Reprod., February 1, 2003; 18(2): 271 - 275.
[Abstract] [Full Text] [PDF]

Home page
 Hum ReprodHome page
M. Ludwig, A. Katalinic, and for the German ICSI Follow-Up Study Group
Pregnancy course and health of children born after ICSI depending on parameters of male factor infertility
Hum. Reprod., February 1, 2003; 18(2): 351 - 357.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.