Human Reproduction, Vol. 17, No. 2, 420-425,
February 2002
© 2002 European Society of Human Reproduction and Embryology
A cytogenetic study of in-vitro matured murine oocytes after ICSI by human sperm
1 Departments of Obstetrics and Gynecology and 2 Biological Science, Asahikawa Medical College, Midorigaoka Higashi 2–1, Asahikawa 0788510, Japan
BACKGROUND: The purpose of this study was to investigate the chromosomal complement and developmental potential of in-vitro matured murine oocytes following ICSI by human sperm. METHODS: Heterologous ICSI fertilization between mouse oocytes and human sperm was employed in order to overcome the reduced fertilization rates observed after conventional IVF due to zona hardening during in-vitro maturation, and to assess separately maternal and paternal chromosome complements. Cytogenetic analyses were performed in four types of oocytes: (i) in-vitro matured metaphase II (MII) oocytes; (ii) in-vivo matured MII oocytes; (iii) in-vitro matured oocytes after ICSI; (iv) in-vivo matured oocytes after ICSI. RESULTS: Activation rates after ICSI of in-vitro matured oocytes was lower than that of in-vivo matured oocytes (69.9 versus 97.2%, P < 0.01), and premature chromosomal condensation was only observed in in-vitro matured oocytes. However, there were no significant differences in developmental rates after successful activation between in-vivo and in-vitro matured ICSI oocytes (69.7 versus 76.6%). The incidences of aneuploidy and structural aberrations were similar between the ICSI embryos and non-ICSI (MII) oocytes. Furthermore, the frequency of chromosomal aberrations was not associated with in-vitro or in-vivo maturation. Similar analyses of paternal chromosomes indicated that there were no significant differences in the incidence of chromosomal aberrations between the embryos derived from in-vitro and in-vivo matured oocytes. CONCLUSIONS: These results suggest that in-vitro matured oocytes following ICSI do not lead to an increase in the frequency of aneuploidy and structural aberrations when human sperm are injected into mouse oocytes.
Key words: chromosomal analysis/human sperm/ICSI/mouse oocytes
3 To whom correspondence should be addressed. E-mail: ksen{at}asahikawa-med.ac.jp
Submitted on December 1, 2000; resubmitted on July 27, 2001
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  Y. Araki, M. Yoshizawa, and Y. Araki A novel method for chromosome analysis of human sperm using enucleated mouse oocytes Hum. Reprod., May 1, 2005; 20(5): 1244 - 1247. [Abstract] [Full Text] [PDF]
|
|