Human Reproduction, Vol. 17, No. 7, 1856-1862,
July 2002
© 2002 European Society of Human Reproduction and Embryology
Embryo quality and IVF treatment outcomes may correlate with different sperm comet assay parameters*
1 Jessop Wing, Royal Hallamshire Hospital, Tree Root Walk, Sheffield S10 2SF, 2 Assisted Conception Unit, St James's University Hospital, Leeds LS7 9TF and 3 Centre For Reproduction, Growth and Development, Level D, Clarendon Wing, Leeds General Infirmary, Leeds LS2 9NS, UK
BACKGROUND: Standard semen parameters have proven poor at predicting the outcomes of IVF treatment cycles. As recent studies suggest that the male genome may play an important role in early embryogenesis, this study attempts to correlate the level of sperm DNA damage in fresh semen and prepared sperm with the outcomes of conventional IVF treatment cycles. METHODS: Forty patients embarking on IVF treatment were recruited into this prospective observational study. Both fresh semen and PureSperm®-prepared sperm were processed using a modified comet assay 3–6 months prior to the patients' IVF treatment cycles. Comet head DNA (mean and integrated head density) and tail DNA parameters (length and moment) were measured separately. RESULTS: Significant correlations between total sperm concentration and between comet length, moment, mean head density with embryo quality were detected in fresh semen and prepared sperm. Surprisingly, no significant correlations between head and tail parameters were detected. CONCLUSIONS: Comet head and tail DNA parameters appear to be potentially useful as predictors of embryo quality and IVF outcomes, especially in couples with unexplained subfertility. The lack of correlation between head and tail parameters may be due to a different mechanism of DNA damage within these two compartments.
Key words: comet assay/embryo quality/IVF/sperm DNA
4 To whom correspondence should be addressed. E-mail: mustitomsu{at}aol.com
* Presented in part at the 17th Annual Meeting of the European Society of Human Reproduction and Embryology, Lausanne, Switzerland on July 1-4, 2001.
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