Human Reproduction, Vol. 17, No. 9, 2338-2343,
September 2002
© 2002 European Society of Human Reproduction and Embryology
Cyclin A1 and gametogenesis in fertile and infertile patients: a potential new molecular diagnostic marker
1 Department of Urology, Universitätsklinikum Benjamin Franklin, Freie Universität Berlin, Berlin, Germany, 2 Department of Medicine, Hematology/Oncology, University of Münster, Münster, Germany, 3 Department of Cell Biology and Biochemistry, Texas Tech University Health Sciences Center, Lubbock, Texas, USA and 4 Department of Andrology, University of Hamburg, Hamburg, Germany
BACKGROUND: The study aim was to evaluate cyclin A1 mRNA expression levels as a potential molecular diagnostic parameter in the work-up of testicular tissue from fertile versus infertile patients. METHODS: Cyclin A1 expression was quantified in 55 cryopreserved testicular tissue specimens by fluorescence real-time RT–PCR. A conventional histological work-up was performed concomitantly in all tissue specimens with additional semi-thin sectioning in all cases of non-obstructive azoospermia (n = 12), maturation arrest (n = 17) and Sertoli cell-only syndrome (SCOS; n = 9). RESULTS: The mean (± SD) normalized cyclin A1 expression (NCyclinA1) was 3.82 ± 2.23 relative gene expression (RGE) in tissue specimens with normal spermatogenesis, and 0.625 ± 0.221 RGE in those with maturation arrest at the level of early spermatids. Only minimal NCyclinA1 was detected in tissue specimens with spermatogonia only or maturation arrest at the level of primary spermatocytes (0.005 ± 0.008). Cyclin A1 expression was absent in the majority of SCOS specimens (0.002 ± 0.002). CONCLUSIONS: These investigations suggested that cyclin A1 expression is altered in cases of spermatogenic disorders. Moreover, the level of cyclin A1 mRNA expression correlates with gametogenic disorders and seems well suited for a molecular-diagnostic classification supplementing the histopathological evaluation of spermatogenic disorders.
Key words: cyclin A1/cyclin-dependent kinase/male fertility/spermatogenesis
5 To whom correspondence should be addressed at: Department of Urology, Universitätsklinikum Benjamin Franklin, Freie Universität Berlin, Hindenburgdamm 30, 12200 Berlin, Germany. E-mail: schrader{at}medizin.fu-berlin.de
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  S.E. Kleiman, L. Yogev, R. Hauser, A. Botchan, B.B.-S. Maymon, G. Paz, and H. Yavetz Expression profile of AZF genes in testicular biopsies of azoospermic men Hum. Reprod., January 1, 2007; 22(1): 151 - 158. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. S. Cheng, P. L. Kuo, Y. N. Teng, T. Y. Kuo, C. L. Chung, Y. H. Lin, R. W. Liao, J. S. N. Lin, and Y. M. Lin Association of spermatogenic failure with decreased CDC25A expression in infertile men Hum. Reprod., September 1, 2006; 21(9): 2346 - 2352. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. M. Lin, Y. N. Teng, C. L. Chung, W. C. Tsai, Y. H. Lin, J. S. N. Lin, and P. L. Kuo Decreased mRNA transcripts of M-phase promoting factor and its regulators in the testes of infertile men Hum. Reprod., January 1, 2006; 21(1): 138 - 144. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Diederichs, N. Baumer, P. Ji, S. K. Metzelder, G. E. Idos, T. Cauvet, W. Wang, M. Moller, S. Pierschalski, J. Gromoll, et al. Identification of Interaction Partners and Substrates of the Cyclin A1-CDK2 Complex J. Biol. Chem., August 6, 2004; 279(32): 33727 - 33741. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 X. Mu, Y.-F. Lee, N.-C. Liu, Y.-T. Chen, E. Kim, C.-R. Shyr, and C. Chang Targeted Inactivation of Testicular Nuclear Orphan Receptor 4 Delays and Disrupts Late Meiotic Prophase and Subsequent Meiotic Divisions of Spermatogenesis Mol. Cell. Biol., July 1, 2004; 24(13): 5887 - 5899. [Abstract] [Full Text] [PDF]
|
|