Human Reproduction, Vol. 18, No. 1, 137-139,
January 2003
© 2003 European Society of Human Reproduction and Embryology
Pregnancy outcome following transfer of human blastocysts vitrified on electron microscopy grids after induced collapse of the blastocoele
Maria Infertility Hospital, Seoul, Korea 1 To whom correspondence should be addressed at: In Vitro Fertilization Laboratory, Maria Infertility Hospital, 103-11, Sinseol-dong, Dongdaemun-gu, Seoul, Korea. e-mail: sonyoung{at}mariababy.com
BACKGROUND: The purpose of this study was to examine the effects on blastocyst survival and subsequent pregnancy rate of ‘artificial shrinkage’ (i.e. induced collapse of the blastocoele) before vitrification of human blastocysts. METHODS: After embryo transfer in IVF cycles, surplus embryos that developed to the expanded blastocyst stage were cryopreserved. Before vitrification on electron microscopy (EM) grids, artificial shrinkage was induced in expanded blastocysts using a 29-gauge needle. After thawing, transfers were performed on 25 couples. Post-thaw survival rates and clinical outcome after the transfer of vitrified blastocysts were examined. RESULTS: Of 90 expanded blastocysts vitrified from 25 patients, 81 survived (90.0%) and 40 of them were hatched (49.4%) at the time of transfer. The implantation rate was 29.0% (20/69), and the pregnancy rate was 48.0% (12/25). Nine patients delivered 15 infants, two pregnancies are ongoing and one ended in miscarriage. CONCLUSIONS: The results suggest that artificial shrinkage is a useful technique for vitrification of expanded blastocysts on EM grids.
Key words: artificial shrinkage/cryopreservation/electron microscopy grid/human blastocyst/vitrification
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  T. Mukaida, C. Oka, T. Goto, and K. Takahashi Artificial shrinkage of blastocoeles using either a micro-needle or a laser pulse prior to the cooling steps of vitrification improves survival rate and pregnancy outcome of vitrified human blastocysts Hum. Reprod., December 1, 2006; 21(12): 3246 - 3252. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W.-Y. Son, S.-Y. Lee, and J.-H. Lim Fertilization, cleavage and blastocyst development according to the maturation timing of oocytes in in vitro maturation cycles Hum. Reprod., November 1, 2005; 20(11): 3204 - 3207. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. Isachenko, M. Montag, E. Isachenko, V. Zaeva, I. Krivokharchenko, R. Shafei, and H. van der Ven Aseptic technology of vitrification of human pronuclear oocytes using open-pulled straws Hum. Reprod., February 1, 2005; 20(2): 492 - 496. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Hiraoka, K. Hiraoka, M. Kinutani, and K. Kinutani Blastocoele collapse by micropipetting prior to vitrification gives excellent survival and pregnancy outcomes for human day 5 and 6 expanded blastocysts Hum. Reprod., December 1, 2004; 19(12): 2884 - 2888. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Sonmezer and K. Oktay Fertility preservation in female patients Hum. Reprod. Update, May 1, 2004; 10(3): 251 - 266. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Hiraoka, K. Hiraoka, M. Kinutani, and K. Kinutani Case report: successful pregnancy after vitrification of a human blastocyst that had completely escaped from the zona pellucida on day 6 Hum. Reprod., April 1, 2004; 19(4): 988 - 990. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Cremades, M. Sousa, J. Silva, P. Viana, S. Sousa, C. Oliveira, J. Teixeira da Silva, and A. Barros Experimental vitrification of human compacted morulae and early blastocysts using fine diameter plastic micropipettes Hum. Reprod., February 1, 2004; 19(2): 300 - 305. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R.P. Cervera and F. Garcia-Ximenez Vitrification of zona-free rabbit expanded or hatching blastocysts: a possible model for human blastocysts Hum. Reprod., October 1, 2003; 18(10): 2151 - 2156. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Vanderzwalmen, G. Bertin, Ch. Debauche, V. Standaert, N. Bollen, E.v. Roosendaal, M. Vandervorst, R. Schoysman, and N. Zech Vitrification of human blastocysts with the Hemi-Straw carrier: application of assisted hatching after thawing Hum. Reprod., July 1, 2003; 18(7): 1504 - 1511. [Abstract] [Full Text] [PDF]
|
|