Human Reproduction, Vol. 18, No. 12, 2654-2659,
December 2003
© 2003 European Society of Human Reproduction and Embryology
Survival of primordial follicles following prolonged transportation of ovarian tissue prior to cryopreservation
1 Laboratory of Reproductive Biology, Section 5712, 2 The Fertility Clinic, Section 4071, University Hospital of Copenhagen, Rigshospitalet, DK-2100 Copenhagen and 3 The Fertility Clinic, University Hospital of Aarhus, Skejby Sygehus, DK-8200 Aarhus, Denmark
4 To whom correspondence should be addressed at: Laboratory of Reproductive Biology, Section 5712, The Juliane Marie Center for Children, Women and Reproduction, Rigshospitalet, Blegdamsvej 9, DK-2100 Copenhagen, Denmark. e-mail: yding{at}rh.dk
BACKGROUND: Cryopreservation of ovarian tissue for fertility preservation is becoming increasingly common. Treatment of diseases that may deprive the ovaries of follicles is often performed at local hospitals that are without the necessary facilities and expertise to cryopreserve ovarian tissue. The aim of the present study was to evaluate whether primordial follicles of ovarian cortex survive transport for up to 4 h prior to cryopreservation. METHODS: Immediately after recovery of one ovary from each of four patients, the cortex was roughly isolated, placed in IVF culture medium, kept on ice and transported for 3–4 h to the centre where final dissection and cryopreservation took place. Transplantation of pieces of thawed ovarian cortex under the skin of ovariectomized immunodeficient mice for a period of 4 weeks was used to assess the survival of primordial follicles. RESULTS: After transplantation, ovarian tissue from each of the four patients contained surviving follicles. CONCLUSIONS: Transport of roughly isolated ovarian cortex cooled on ice for a period of up to 4 h allows survival of primordial follicles following cryopreservation and transplantation to immunodeficient mice.
Key words: cryopreservation/ovary/survival of follicles/transportation/xenotransplantation
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