Human Reproduction, Vol. 18, No. 5, 1023-1028,
May 2003
© 2003 European Society of Human Reproduction and Embryology
Sperm DNA fragmentation decreases the pregnancy rate in an assisted reproductive technique
1 Département de Médecine de la Reproduction, Hôpital Edouard Herriot, Place d’Arsonval, 69373 Lyon cedex 08, 2 INSERM U418, Hôpital Debrousse, 29 Rue Soeur Bouvier, 69322 Lyon cedex 05, France
3 To whom correspondence should be addressed at: Laboratoire de Biologie de la Reproduction, 8 avenue Rockefeller, 69373 Lyon Cedex 08, France. e-mail: benchaib{at}rockefeller.univ-lyon1.fr
BACKGROUND: Standard sperm characteristics are poor predictors of the outcome of IVF treatments. On the contrary, sperm genome quality has been emphasized for several years as playing a major role in early embryogenesis, thus in the success of IVF attempt. METHODS: Sperm DNA fragmentation from a selected group of 104 couples undergoing assisted reproductive techniques (ART) (IVF: n = 50; and ICSI: n = 54) was measured by TUNEL assay and correlated with semen and ART outcomes. RESULTS: A negative correlation was found between sperm characteristics and the proportion of sperm showing DNA fragmentation. For fragmentation >10%, a significant decrease of the fertilization rate was observed. No correlation was found between sperm DNA fragmentation and embryo quality. A high proportion of sperm with fragmented DNA was a pejorative factor to obtain pregnancies when ICSI was performed, but there was no relationship when conventional IVF was performed. CONCLUSIONS: The proportion of sperm with DNA fragmentation appears to be potentially useful as a predictor of ICSI outcome, whereas embryo quality based on morphological criteria, appeared unaffected by DNA fragmentation.
Key words: ART/DNA fragmentation/embryo quality/human sperm
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