Human Reproduction, Vol. 18, No. 9, 1908-1917,
September 2003
© 2003 European Society of Human Reproduction and Embryology
Laboratory Investigations |
Meiosis-activating sterol protects oocytes from precocious chromosome segregation*
1 Research Laboratories of Schering AG, Berlin and 2 Fakultät für Biologie, Gentechnologie/Biotechnologie, University Bielefeld, Bielefeld, Germany
3 To whom correspondence should be addressed at: University of Bielefeld, Universitätsstrasse 25, 33501 Bielefeld, Germany. e-mail: Suna.Cukurcam{at}Uni-Bielefeld.de
BACKGROUND: Follicular fluid meiosis-activating sterol (FF-MAS) overcomes hypoxanthine (HX)-mediated meiotic arrest in mammalian oocytes. METHODS: In order to determine whether chromosome segregation was normal in oocytes matured in FF-MAS, the development, chromosomal constitution and chromosome alignment was analysed in spontaneously matured as well as HX-arrested mouse oocytes cultured in the absence or presence of FF-MAS. RESULTS: FF-MAS-induced meiotic maturation was significantly less effective compared with spontaneous maturation in supporting cytokinesis (
40 and 90% polar body formation respectively). The majority of oocytes stimulated by FF-MAS to overcome the HX block developed to metaphase II (MII), but 23.4% of meiosis II oocytes were diploid. Chromosomes were well aligned on the spindle, and hyperploidy was low in spontaneously matured oocytes and HX-arrested oocytes cultured with or without FF-MAS. Unexpectedly, almost 40% of spontaneously matured MII oocytes contained chromatids/monads. Precocious loss of chromatid cohesion was significantly reduced in spontaneously matured as well as HX-arrested oocytes cultured in the presence of FF-MAS but not lanosterol. CONCLUSIONS: FF-MAS induces full nuclear maturation to MII, and chromosomes segregate with high fidelity. However, in delayed FF-MAS-stimulated meiotic maturation, anaphase I may occur in the absence of cytokinesis. FF-MAS appears to protect mammalian oocytes from precocious chromatid segregation.
Key words: maturation/meiosis/mouse/non-disjunction/oocyte
* Preliminary results were presented at the 18th Annual Meeting of the European Society of Human Reproduction and Embryology, Vienna, 2002.
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