Human Reproduction, Vol. 19, No. 1, 118-123,
January 2004
© 2004 European Society of Human Reproduction and Embryology
Analysis of chromosomal abnormalities in testicular and epididymal spermatozoa from azoospermic ICSI patients by fluorescence in-situ hybridization
1 Instituto Valenciano de Infertilidad, Valencia and Departments of 2 Pediatrics, Obstetrics and Gynecology and 3 Surgery, University of Valencia, Valencia, Spain
4 To whom correspondence should be addressed at: Instituto Valenciano de Infertilidad, Plaza Policía Local 3, 46015 Valencia, Spain. e-mail: Manuel.Gil-salom{at}uv.es
BACKGROUND: An increased incidence of numerical chromosomal abnormalities has been reported in the ejaculated spermatozoa of infertile patients. However, there are few cytogenetic studies of testicular and epididymal spermatozoa, and their results are still controversial. METHODS: Fluorescence in-situ hybridization (FISH) analysis of chromosomes 13, 18, 21, X and Y was performed on seven testicular samples and two epididymal samples from patients with obstructive azoospermia (OA), and on 13 testicular samples from patients with non-obstructive azoospermia (NOA). Five ejaculated sperm samples from normozoospermic fertile donors were evaluated as a control group. RESULTS: Both epididymal sperm samples showed normal FISH results for the parameters analysed when compared with those of the control group. FISH results were abnormal in 29% (two of seven) of testicular samples from OA patients and in 54% (seven of 13) of those from NOA patients, although this difference was not statistically significant. Testicular samples from OA patients showed a significant increase of disomy for sex chromosomes (P < 0.01), whereas NOA patients displayed significantly higher rates of diploidy (P < 0.0001) and disomy for chromosomes 13 (P < 0.0001), 21 (P < 0.001) and sex chromosomes (P < 0.0001) than the control group. CONCLUSIONS: Testicular spermatozoa from azoospermic patients present increased rates of chromosomal abnormalities, mainly of the sex chromosomes, which are particularly high in NOA patients.
Key words: azoospermia/chromosomal abnormality/epididymal spermatozoa/fluorescence in-situ hybridization/testicular spermatozoa
Submitted June 9, 2003 ; accepted September 23, 2003
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  K. A. Ferguson, E. C. Wong, V. Chow, M. Nigro, and S. Ma Abnormal meiotic recombination in infertile men and its association with sperm aneuploidy Hum. Mol. Genet., December 1, 2007; 16(23): 2870 - 2879. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Ciriminna, M.L. Papale, P.G. Artini, M. Costa, L. De Santis, L. Gandini, L. Parmegiani, G. Ragni, A. Revelli, L. Rienzi, et al. Impact of Italian legislation regulating assisted reproduction techniques on ICSI outcomes in severe male factor infertility: a multicentric survey Hum. Reprod., September 1, 2007; 22(9): 2481 - 2487. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. E. Marchesi and H. L. Feng Sperm DNA Integrity From Sperm to Egg J Androl, July 1, 2007; 28(4): 481 - 489. [Full Text] [PDF]
|
|
|
|
|
|
E. Mateu, L. Rodrigo, N. Prados, M. Gil-Salom, J. Remohi, A. Pellicer, and C. Rubio High Incidence of Chromosomal Abnormalities in Large-Headed and Multiple-Tailed Spermatozoa J Androl, January 1, 2006; 27(1): 6 - 10. [Full Text] [PDF]
|
|
|
|
|
|
T. Viloria, M.C. Rubio, L. Rodrigo, G. Calderon, A. Mercader, E. Mateu, M. Meseguer, J. Remohi, and A. Pellicer Smoking habits of parents and male: female ratio in spermatozoa and preimplantation embryos Hum. Reprod., September 1, 2005; 20(9): 2517 - 2522. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Araki, M. Yoshizawa, and Y. Araki A novel method for chromosome analysis of human sperm using enucleated mouse oocytes Hum. Reprod., May 1, 2005; 20(5): 1244 - 1247. [Abstract] [Full Text] [PDF]
|
|