Skip Navigation

This Article
Right arrow Full Text Freely available
Right arrow FREE Full Text (PDF ) Freely available
Right arrow Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (14)
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Demir, A.Y.
Right arrow Articles by Dunselman, G.A.J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Demir, A.Y.
Right arrow Articles by Dunselman, G.A.J.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Human Reproduction, Vol. 19, No. 1, 21-29, January 2004
© 2004 European Society of Human Reproduction and Embryology

Menstrual effluent induces epithelial–mesenchymal transitions in mesothelial cells

A.Y. Demir1,3, P.G. Groothuis1,3,4, A.W. Nap1,3, C. Punyadeera2,3, A.F.P.M.de Goeij2,3, J.L.H. Evers1,3 and G.A.J. Dunselman1,3

Departments of 1 Obstetrics & Gynaecology and 2 Pathology, 3 Research Institute Growth and Development (GROW), Academisch ziekenhuis Maastricht and Maastricht University, Maastricht, The Netherlands

4 To whom correspondence should be addressed at: Department of Pathology, Academic Hospital Maastricht, Peter Debyelaan 25, Postbus 5800, 6202 AZ, Maastricht, The Netherlands. e-mail: Patrick.Groothuis{at}path.unimaas.nl

BACKGROUND: Menstrual effluent affects mesothelial cell (MC) morphology. We evaluated whether these changes were consistent with epithelial–mesenchymal transitions (EMT). METHODS: Monolayer cultures of MC were incubated overnight in conditioned media, prepared from cells isolated form menstrual effluent, with or without kinase and ATP inhibitors. Changes in cell morphology were monitored using time-lapse video microscopy and immunohistochemistry. Effects on the expression of EMT-associated molecules were evaluated using real-time RT–PCR and/or Western blot analysis. RESULTS: Incubation in conditioned media disrupted cell–cell contacts, and increased MC motility. The changes were reversible. During the changes the distribution of cytokeratins, fibrillar actin and {alpha}-tubulin changed. Sodium azide, an inhibitor of ATP production, and Genistein, a general tyrosine kinase inhibitor, antagonized these effects. Wortmannin, a phosphatidylinositol 3-kinase inhibitor, and SU6656, an Src tyrosine kinase inhibitor, only partially antagonized the effect. The expression of Snail and vimentin was markedly up-regulated, whereas the expression of E-cadherin was decreased and cytokeratins were altered. CONCLUSIONS: In MC, menstrual effluent initiates a reversible, energy-dependent transition process from an epithelial to a mesenchymal phenotype. Involvement of the (Src) tyrosine kinase signalling pathway and the changes in the expression of cytokeratins, Snail, vimentin and E-cadherin demonstrate that the morphological changes are EMT.

Key words: E-cadherin/endometriosis/mesothelial cells/Snail/vimentin


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Hum Reprod UpdateHome page
E. M. C. Ohlsson Teague, C. G. Print, and M. L. Hull
The role of microRNAs in endometriosis and associated reproductive conditions
Hum. Reprod. Update, September 22, 2009; (2009) dmp034v1.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
M. Chen, H. Cai, J.-L. Yang, C.-L. Lu, T. Liu, W. Yang, J. Guo, X.-Q. Hu, C.-H. Fan, Z.-Y. Hu, et al.
Effect of Heat Stress on Expression of Junction-Associated Molecules and Upstream Factors Androgen Receptor and Wilms' Tumor 1 in Monkey Sertoli Cells
Endocrinology, October 1, 2008; 149(10): 4871 - 4882.
[Abstract] [Full Text] [PDF]

Home page
 Mol Hum ReprodHome page
S. Defrere, J.C. Lousse, R. Gonzalez-Ramos, S. Colette, J. Donnez, and A. Van Langendonckt
Potential involvement of iron in the pathogenesis of peritoneal endometriosis
Mol. Hum. Reprod., July 1, 2008; 14(7): 377 - 385.
[Abstract] [Full Text] [PDF]

Home page
 Physiol. GenomicsHome page
J. C. Sluimer, N. Kisters, K. B. Cleutjens, O. L. Volger, A. J. Horrevoets, L. H. van den Akker, A.-P. J. Bijnens, and M. J. Daemen
Dead or alive: gene expression profiles of advanced atherosclerotic plaques from autopsy and surgery
Physiol Genomics, August 20, 2007; 30(3): 335 - 341.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
N. Ahmed, S. Maines-Bandiera, M. A. Quinn, W. G. Unger, S. Dedhar, and N. Auersperg
Molecular pathways regulating EGF-induced epithelio-mesenchymal transition in human ovarian surface epithelium
Am J Physiol Cell Physiol, June 1, 2006; 290(6): C1532 - C1542.
[Abstract] [Full Text] [PDF]

Home page
 DevelopmentHome page
A. Barrallo-Gimeno and M. A. Nieto
The Snail genes as inducers of cell movement and survival: implications in development and cancer
Development, July 15, 2005; 132(14): 3151 - 3161.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.