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Hum. Reprod. Advance Access originally published online on August 6, 2004
Human Reproduction 2004 19(10):2345-2349; doi:10.1093/humrep/deh442
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Human Reproduction vol. 19 no. 10 © European Society of Human Reproduction and Embryology 2004; all rights reserved

Effects of sucrose concentration on the developmental potential of human frozen–thawed oocytes at different stages of maturity

Z.J. Chen1,2, M. Li1, Y. Li1, L.X. Zhao1, R. Tang1, Y. Sheng1, X. Gao1, C.H. Chang2 and H.L. Feng3,4

1 Center for Reproductive Medicine, Shandong Provincial Hospital, Shandong University, Jinan 250021, China, 2 Department of Medicine, Case Western Reserve University, Cleveland, Ohio 44106 and 3 Center for Human Reproduction, North Shore University Hospital, NYU School of Medicine, Manhasset, NY 11030, USA

4 To whom correspondence should be addressed at: Center for Human Reproduction, North Shore University Hospital, 300 Community Drive, Manhasset, NY 11030, USA. Email: hfeng{at}nshs.edu

BACKGROUD: Success of human oocyte cryopreservation depends on multiple cryobiological factors that could influence the developmental potential of the oocytes. The objective of this study was to examine the effects of different sucrose concentrations on the developmental potential of human frozen–thawed oocytes at different maturity stages. METHODS: A total of 355 oocytes collected from small follicles were randomly divided into three groups and two groups (B and C) were cryopreserved using slow-freezing method. Group A included 131 oocytes at different maturity stages without freezing. Another 119 oocytes in Group B were cryopreserved with 0.1 M sucrose and 105 oocytes in Group C with 0.2 M sucrose concentration. RESULTS: The post-thaw survival rate of the oocytes and the cleavage rate in Group C were significantly higher than that of Group B (P<0.05). For immature metaphase I (MI) stage oocytes, a significant difference was found in the maturation rate between Group C and Group B (P<0.05). The maturation rate for the GV oocytes in Groups A and C was significantly higher than Group B (P<0.01). CONCLUSIONS: The results suggested that sucrose concentration of 0.2 M in the cryoprotectant solution is more suitable for human oocyte cryopreservation.

Key words: cryopreservation/embryo/human fertilization in vitro/ooctyes


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