Hum. Reprod. Advance Access originally published online on March 25, 2004
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Human Reproduction, Vol. 19, No. 5, 1140-1147,
May 2004
© 2004 European Society of Human Reproduction and Embryology
Reduced oocyte activation and first cleavage rate after ICSI with spermatozoa from a sterile mouse chromosome mutant
1 Laboratory of Genetics, Wageningen Institute of Animal Sciences, Wageningen University, 6701BH Wageningen, The Netherlands, 2 Department of Obstetrics and Gynaecology, University Medical Centre St Radboud, 6500HB Nijmegen, The Netherlands, 3 Experimental Animal Centre, 6703HD Wageningen University, The Netherlands and 4 Institute of Reproductive Medicine of the University of Münster, D-48129 Münster, Germany.
5 Current address: Division of Reproductive Medicine, Department of Obstetrics and Gynaecology, Erasmus Medical Center, 3015 GD Rotterdam, The Netherlands.
6 Current address: Department of Obstetrics and Gynaecology, University Medical Centre St Radboud, 6500HB Nijmegen, The Netherlands. To whom correspondence should be addressed at: Department of Obstetrics and Gynaecology, UMCN St Radboud, 415 OBGYN, PO Box 9101, 6500HB Nijmegen, The Netherlands. e-mail: P.deBoer{at}Obgyn.UMCN.nl
BACKGROUND: Male mice, heterozygous for two semi-identical reciprocal translocations T(1;13)70H and T(1;13)1Wa are usually sterile. We have investigated this oligoasthenoteratozoospermic mouse model using ICSI. METHODS: B6D2F1 oocytes were injected with epididymal or testicular sperm from fertile or sterile translocation carriers and from chromosomally normal fertile controls. ICSI efficiency was determined by pronucleus formation and first cleavage rates. For arrested zygotes, cell cycle progression was evaluated by BrdU incorporation and incubation with okadaic acid. RESULTS: Epididymal sperm from infertile translocation carriers showed a slightly lower fertilization rate (70% vs. 92%, 95% and 95% for fertile translocation carriers and two groups of normal fertile control males, respectively) and a severely reduced cleavage rate (33% vs. 87%, 96% and 89% for the same control groups). However, the use of testicular sperm significantly improved the cleavage rate (62% vs. 83% for normal fertile controls). Development of arrested zygotes was delayed or blocked during S- and G2-phase. CONCLUSIONS: Whereas control testicular and epididymal sperm performed equally well, the use of testicular sperm from oligospermic T/T' males significantly increased first cleavage rates when compared to the low rates with epididymal sperm. Epididymal storage in oligospermics may negatively influence zygote division.
Key words: DNA damage/ICSI/male sterility/mouse/zygote
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