Hum. Reprod. Advance Access originally published online on April 29, 2004
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Human Reproduction, Vol. 19, No. 6, 1401-1408,
June 2004
© 2004 European Society of Human Reproduction and Embryology
The predictive value of sperm chromatin structure assay (SCSA) parameters for the outcome of intrauterine insemination, IVF and ICSI
1 Fertility Clinic, Viborg Hospital (Skive), Resenvej 25, DK 7800 Skive, Denmark, 2 Section of Toxicology and Biomedical Sciences, BIOTEC-MED, ENEA Casaccia Research Center, Rome, Italy and 3 Fertility Centre, Scanian Andrology Centre, Malmö University Hospital, Malmö, Sweden
4 To whom correspondence should be addressed. e-mail: mona.bungum{at}sygehusviborg.dk
INTRODUCTION: Sperm chromatin integrity assessment has been suggested as a fertility predictor. The aim of this study was to examine the relationship between the results of sperm chromatin structure assay (SCSA) and the outcome of IVF, ICSI and intrauterine insemination (IUI). METHODS: A total of 306 consecutive couples undergoing assisted reproduction were included. IUI was performed in 131, IVF in 109 and ICSI in 66. SCSA results were expressed as DNA fractionation index (DFI) and highly DNA stainable (HDS) cell fractions. Reproductive outcome parameters were biochemical pregnancy (BP), clinical pregnancy (CP) and delivery (D). RESULTS: For IUI, the chance of pregnancy/delivery was significantly higher in the group with DFI 
27% and HDS 10% than in patients with DFI >27% or HDS >10%. The odds ratios (ORs) (95% confidence intervals) were 20 (2.3–117), 16 (1.9–137) and 14 (1.6–110) for BP, CP and D, respectively. No statistical difference between the outcomes of IVF versus ICSI was observed in the group with DFI 27%. In the DFI >27% group, however, the results of ICSI were significantly better than those of IVF. Comparing ICSI with IVF, the OR (95% CI) for BP was 26 (1.9–350). CONCLUSIONS: SCSA is a useful method for prediction of the outcome of assisted reproduction.
Key words: ART/DNA damage/pregnancy rate/sperm chromatin
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