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Hum. Reprod. Advance Access originally published online on June 3, 2004
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Human Reproduction, Vol. 19, No. 7, 1562-1569, July 2004
© 2004 European Society of Human Reproduction and Embryology

Establishment of a capillary–cumulus model to study the selection of sperm for fertilization by the cumulus oophorus

S.J. Hong1,2, P.C. Chiu1, K.F. Lee1, J.M.Y. Tse1,3, P.C. Ho1 and W.S.B. Yeung1,4

1 Department of Obstetrics and Gynaecology, University of Hong Kong, Queen Mary Hospital, Pokfulam, Hong Kong, China

2 Present address: Department of Obstetrics and Gynaecology, Second Affiliated Hospital, Sun Yat-sen University, 107 West Yan Jiang Road, Guangzhou 510120, Guangdong, China

3 Present address: Innovation and Technology Commission, 14/F, Ocean Centre, 5 Canton Road, Kowloon, Hong Kong, China

4 To whom correspondence should be addressed. e-mail: wsbyeung{at}hkucc.hku.hk

BACKGROUND: Spermatozoa have to traverse the cumulus oophorus before fertilization in vivo. Evidence suggests that the cumulus oophorus plays an important role in the fertilization process. We describe the establishment of a capillary–cumulus oophorus model with which to study the action of cumulus mass on the function of human spermatozoa. METHODS: Human cumulus oophorus was aspirated into a glass capillary. Spermatozoa were allowed to pass through the cumulus mass in the capillary from one end of the capillary. The spermatozoa that had traversed the mass were collected at the other end of the capillary and underwent sperm function analyses. RESULTS: Compared with those spermatozoa cultured in medium alone, spermatozoa exposed to the cumulus mass were more likely to have normal morphology, be capacitated and acrosome reacted, with a distinct motility pattern and better zona-binding capacity. CONCLUSION: A novel in vitro model for spermatozoa penetration through the cumulus oophorus was established. The model can be applied to investigate the effect of the cumulus oophorus on sperm functions.

Key words: cumulus oophorus/human spermatozoa


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