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Hum. Reprod. Advance Access originally published online on April 29, 2004
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Human Reproduction, Vol. 19, No. 7, 1604-1607, July 2004
© 2004 European Society of Human Reproduction and Embryology

Pregnancy following chemical activation of oocytes in a couple with repeated failure of fertilization using ICSI: Case report

Yoshihiko Murase1,5, Yasuyuki Araki2, Satoshi Mizuno3, Choji Kawaguchi1, Mayumi Naito1, Midori Yoshizawa4 and Yasuhisa Araki2

1 Kanayama Lady’s Clinic, Meganeplaza Bldg. 6F, 1-201, Kanayama-cho, Atsuta-ku, Nagoya, 456-0002, 2 The Institute for ARMT, 909-21, Ishii, Fujimi, Setagun, Gunma, 371-0105, 3 IVF Osaka Clinic, 2-2-30, Nagatanaka, Higashi-Osaka-shi, Osaka, 550-0015 and 4 Department of Animal Breeding and Reproduction, Faculty of Agriculture, Utsunomiya University, 350, Mine-machi, Utsunomiya, Tochigi, 321-8505, Japan

5 To whom correspondence should be addressed. e-mail: murase{at}klc.ne.jp

We report our attempts to achieve a successful pregnancy outcome with calcium ionophore A23187 and puromycin oocyte activation using sperm from a normozoospermic husband of a patient with previous repeated failed fertilization following ICSI. Oocytes from the female partner of a couple with a 4 year history of unexplained primary infertility with repeated failed fertilization following ICSI were used. In the latest ICSI attempt, oocytes were activated by treatment with calcium ionophore (5 min) and puromycin (5 h), then cultured. In this cycle, assisted oocyte activation with calcium ionophore and puromycin after ICSI resulted in a satisfactory fertilization rate (8/12; 66.7%); in prior cycles only one of 71 oocytes (1.4%) was fertilized. The outcome was a Caesarean section delivery of a healthy male infant without congenital abnormalities at 41 weeks, 2 days of gestation. In conclusion, the use of calcium ionophore and puromycin for oocyte activation was found to be a useful method in a case of repeated failed fertilization after ICSI.

Key words: calcium ionophore/failed fertilization/ICSI/oocyte activation/puromycin


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