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Human Reproduction, Vol. 2, No. 7, pp. 573-575, 1987
© 1987 European Society of Human Reproduction and Embryology

research-article

Sexing of human sperm by discontinuous Percoll density gradient and its clinical application

Rihachi Iizuka, Satoru Kaneko, Rui Aoki and Toshifumi Kobayashi

Department of Obstetrics and Gynaecology, School of Medicine, Keio University 35 Shinanomachi, Shinjuku-ku, Tokyo 160, Japan

A discontinuous Percoll density gradient was employed for the selective isolation of human X-bearing sperm. The fraction rich in X-bearing sperm that sedimented through eight steps of a Percoll density gradient was inseminated into healthy volunteers. Six couples achieved pregnancy and each delivered a female baby without abnormality. For further separation, a Percoll density gradient consisting of 12 steps gave a 94% purification of X-bearing sperm (contamination with Y-bearing sperm: 6.4 ± 1.8%) with a mean recovery of 23.3 ± 6.4% (n = 6).


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