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Human Reproduction, Vol. 2, No. 7, pp. 583-587, 1987
© 1987 European Society of Human Reproduction and Embryology

research-article

Study of human sperm chromosomes by sequential transmission and scanning electron microscopy

J. Navarro1,2,3, J. Benet1, A. Genescà1, N. Castell4, J. Egozcue1,2 and C. Templado1,2

1Departarnent de Biologia Cellular i Fisiologia, Subunitat de Biologia de la Facultat de Medicina 2Institut de Biologia Fonamental ‘V. Villar Palasi’ 4Servei de Microscòpia Electrònica, Universitat Autònoma de Barcelona 08193 Bellaterra, Spain

Correspondence: 3To whom correspondence should be addressed

We describe a method of observing human sperm metaphases by sequential transmission and scanning electron microscopy. This permits the analysis of ultrastructural aspects of sperm chromosomes and allows the relationship between ultrastnicture, heterochromatin condensation, and the behaviour and staining properties of sperm chromosomes and heterochromatic regions to be determined.

Key words: heterochromatin condensation/human sperm metaphases/ultrastructure/t.e.m./s.e.m.


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