Hum. Reprod. Advance Access originally published online on July 8, 2005
Human Reproduction 2005 20(10):2715-2723; doi:10.1093/humrep/dei156
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Reproductive biology |
Interleukin-6- and tumour necrosis factor 
-mediated expression of hepatocyte growth factor by stromal cells and its involvement in the growth of endometriosis
1 Department of Obstetrics and Gynecology, 2 Department of Molecular Pathology, Atomic Bomb Disease Institute, Nagasaki University, 3 Division of Cytokine Signaling, Department of Molecular Microbiology and Immunology, Graduate School of Biomedical Sciences, Nagasaki University, Nagasaki, Japan
4 To whom correspondence should be addressed. Department of Obstetrics and Gynecology, Graduate School of Biomedical Sciences, Nagasaki University, 1-7-1 Sakamoto, Nagasaki 852-8501, Japan. E-mail: nemokhan{at}net.nagasaki-u.ac.jp
BACKGROUND: We investigated the expression of the hepatocyte growth factor (HGF) gene and protein by the stromal cells derived from women with or without endometriosis and its regulation by interleukin-6 (IL-6) and tumour necrosis factor 
(TNF). METHODS: Stromal cells immunoreactive to vimentin were isolated from the eutopic and ectopic endometrium of 18 infertile women with endometriosis and 12 women without endometriosis. The production of HGF in the culture media of basal and IL-6- or TNF-stimulated stromal cells was examined with an enzyme-linked immunosorbent assay. The mRNA expression of HGF and its receptor c-Met in the stroma was investigated by RT–PCR. The localization of HGF and c-Met in isolated stromal cells and in intact tissue was examined by immunohistochemistry. The effect of HGF on the growth of stromal cells alone or in combination with IL-6 or TNF was examined in a bromodeoxyuridine (BrdU) incorporation study. RESULTS: The production of HGF in the culture medium of stromal cells was significantly increased after single or combined treatment with either IL-6 or TNF when compared with non-treated cells. The production of HGF by stromal cells derived from the eutopic endometrium of women with endometriosis was significantly higher than that of cells from women without endometriosis. This effect was paralleled by increased expression of HGF and c-Met mRNA, as demonstrated by RT–PCR. The BrdU incorporation study indicated that the addition of HGF enhanced the growth of endometrial and endometriotic stroma alone or in combination with IL-6 or TNF. CONCLUSION: IL-6 and TNF are involved in the production of HGF by endometrial stromal cells and may be involved in the growth of endometriosis by an autocrine mechanism.
Key words:
cell growth/endometriosis/hepatocyte growth factor/IL-6/TNF
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  K. N. Khan, M. Kitajima, T. Imamura, K. Hiraki, A. Fujishita, I. Sekine, T. Ishimaru, and H. Masuzaki Toll-like receptor 4-mediated growth of endometriosis by human heat-shock protein 70 Hum. Reprod., July 1, 2008; (2008) den195v1. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 O. Guzeloglu-Kayisli, G. Halis, S. Taskiran, U. A. Kayisli, and A. Arici DNA-Binding Ability of NF-{kappa}B is Affected Differently by ER{alpha} and ER{beta} and Its Activation Results in Inhibition of Estrogen Responsiveness Reproductive Sciences, May 1, 2008; 15(5): 493 - 505. [Abstract] [PDF]
|
|
|
|
|
|
J. D. Wren, Y. Wu, and S.-W. Guo A system-wide analysis of differentially expressed genes in ectopic and eutopic endometrium Hum. Reprod., August 1, 2007; 22(8): 2093 - 2102. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. Skibinski, J. S. Elborn, and M. Ennis Bronchial epithelial cell growth regulation in fibroblast cocultures: the role of hepatocyte growth factor Am J Physiol Lung Cell Mol Physiol, July 1, 2007; 293(1): L69 - L76. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 W. L. Bigbee, J. R. Grandis, and J. M. Siegfried Multiple Cytokine and Growth Factor Serum Biomarkers Predict Therapeutic Response and Survival in Advanced-Stage Head and Neck Cancer Patients Clin. Cancer Res., June 1, 2007; 13(11): 3107 - 3108. [Full Text] [PDF]
|
|
|
|
|
|
J.-H. Yang, M.-Y. Wu, C.-D. Chen, M.-J. Chen, Y.-S. Yang, and H.-N. Ho Altered apoptosis and proliferation in endometrial stromal cells of women with adenomyosis Hum. Reprod., April 1, 2007; 22(4): 945 - 952. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Miura, K. N. Khan, M. Kitajima, K. Hiraki, S. Moriyama, H. Masuzaki, T. Samejima, A. Fujishita, and T. Ishimaru Differential infiltration of macrophages and prostaglandin production by different uterine leiomyomas Hum. Reprod., October 1, 2006; 21(10): 2545 - 2554. [Abstract] [Full Text] [PDF]
|
|