Skip Navigation


Hum. Reprod. Advance Access originally published online on June 24, 2005
Human Reproduction 2005 20(10):2724-2735; doi:10.1093/humrep/dei140
This Article
Right arrow Full Text Freely available
Right arrow FREE Full Text (PDF ) Freely available
Right arrow All Versions of this Article:
20/10/2724    most recent
dei140v1
Right arrow Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (9)
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Jones, R. L.
Right arrow Articles by Salamonsen, L. A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Jones, R. L.
Right arrow Articles by Salamonsen, L. A.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

© The Author 2005. Published by Oxford University Press on behalf of the European Society of Human Reproduction and Embryology. All rights reserved. For Permissions, please email: journals.permissions@oupjournals.org

Reproductive biology

Chemokine expression is dysregulated in the endometrium of women using progestin-only contraceptives and correlates to elevated recruitment of distinct leukocyte populations

Rebecca L. Jones1, Naomi B. Morison1,4, Natalie J. Hannan1,2, Hilary O.D. Critchley3 and Lois A. Salamonsen1

1 Prince Henry’s Institute of Medical Research, PO Box 5152, Clayton, Victoria 3168, 2 Department of Obstetrics & Gynaecology, Monash University, Clayton, Victoria 3168, Australia and 3 Department of Reproductive and Developmental Sciences, University of Edinburgh, Centre for Reproductive Biology, Edinburgh, UK

4 To whom correspondence should be addressed. E-mail: naomi.morison{at}phimr.monash.edu.au
R.L.Jones and N.B.Morison contributed equally to this work

BACKGROUND: Breakthrough bleeding (BTB) is the most common reason for discontinuation of progestin-only (p-only) contraceptives, yet the causes are unknown. Use of p-only contraceptives is associated with elevated influx of endometrial leukocytes, similar to that observed perimenstrually or within decidualized endometrium. We hypothesized that chemokine expression is altered in women using p-only contraceptives, leading to abnormal leukocyte recruitment and BTB. METHODS: Expression of eight highly abundant endometrial chemokines was examined using immunohistochemistry and real-time PCR, in endometria from women using subdermal and intrauterine levonorgestrel and correlated to leukocyte subpopulations. RESULTS: Macrophage-derived chemokine (MDC), hemofiltrate CC chemokine-1 (HCC-1), monocyte chemoattractant protein-3 (MCP-3), interleukin-8 (IL-8) and eotaxin were strongly produced by epithelial glands, comparable to levels in premenstrual phase endometrium. Stromal cells were negative for chemokines in atrophic/shedding endometria, but intensely positive in highly decidualized tissues for MDC, MCP-3, HCC-1 and 6Ckine. Macrophage inflammatory protein-1{beta} (MIP-1b) and HCC-4 were suppressed in all p-exposed endometria. CONCLUSIONS: These data demonstrate that chemokine expression is dysregulated in p-exposed endometria, consistent with the morphological appearance of the endometrium and the leukocyte subsets present. This reinforces a potential role for chemokines in the elevated leukocyte recruitment that contributes to endometrial fragility and BTB.

Key words: chemokines/contraceptives/endometrium/leukocytes/progestin


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Reproductive SciencesHome page
C. J. Lockwood, G. Krikun, M. Hickey, S. J. Huang, and F. Schatz
Decidualized Human Endometrial Stromal Cells Mediate Hemostasis, Angiogenesis, and Abnormal Uterine Bleeding
Reproductive Sciences, February 1, 2009; 16(2): 162 - 170.
[Abstract] [PDF]

Home page
 Hum ReprodHome page
N. Chegini, X. Luo, Q. Pan, A. Rhoton-Vlasak, and D.F. Archer
Endometrial expression of epithelial neutrophil-activating peptide-78 during the menstrual cycle or in progestin-only contraceptive users with breakthrough bleeding and the influence of doxycycline therapy
Hum. Reprod., February 1, 2007; 22(2): 427 - 433.
[Abstract] [Full Text] [PDF]

Home page
 Hum ReprodHome page
R. Li, X. Luo, Q. Pan, I. Zineh, D. F. Archer, R.S. Williams, and N. Chegini
Doxycycline alters the expression of inflammatory and immune-related cytokines and chemokines in human endometrial cells: implication in irregular uterine bleeding
Hum. Reprod., October 1, 2006; 21(10): 2555 - 2563.
[Abstract] [Full Text] [PDF]

Home page
 Hum ReprodHome page
A. Peloggia, C. A. Petta, L. Bahamondes, M. Oliveira-Ribeiro, J. Zhang, and L. Salamonsen
Endometrial chemokines, uterine natural killer cells and mast cells in long-term users of the levonorgestrel-releasing intrauterine system
Hum. Reprod., May 1, 2006; 21(5): 1129 - 1134.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
X.-H. Ma, S.-J. Hu, H. Ni, Y.-C. Zhao, Z. Tian, J.-L. Liu, G. Ren, X.-H. Liang, H. Yu, P. Wan, et al.
Serial Analysis of Gene Expression in Mouse Uterus at the Implantation Site
J. Biol. Chem., April 7, 2006; 281(14): 9351 - 9360.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.