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Hum. Reprod. Advance Access originally published online on June 24, 2005
Human Reproduction 2005 20(10):2736-2744; doi:10.1093/humrep/dei125
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© The Author 2005. Published by Oxford University Press on behalf of the European Society of Human Reproduction and Embryology. All rights reserved. For Permissions, please email: journals.permissions@oupjournals.org

Reproductive biology

Short-term ischaemic storage of human uterine myometrium—basic studies towards uterine transplantation

C.Almén Wranning1,4, J. Mölne2, R.Racho El-Akouri1, G. Kurlberg3 and M. Brännström1

1 Department of Obstetrics and Gynecology, 2 Department of Clinical Pathology and Cytology, and 3 Department of Surgery, The Sahlgrenska Academy at Göteborg University, Sahlgrenska University Hospital, S-413 45 Göteborg, Sweden

4 To whom correspondence should be addressed at: Department of Obstetrics and Gynecology, Institute for the Health of Women and Children, The Sahlgrenska Academy at Göteborg University, Sahlgrenska University Hospital, S-413 45 Göteborg, Sweden. E-mail: caiza.wranning{at}obgyn.gu.se

BACKGROUND: Transplantation of the uterus has been suggested as a possible future treatment of absolute uterine infertility. The tolerability of human uterine tissue to cold ischaemic storage was tested in the present study. METHODS: Small tissue samples of human uteri were subjected to cold (4°C) ischaemia (6 and 24 h) in Ringer acetate (RIN), the intracellular-like University of Wisconsin solution (UW) or the extracellular-like Perfadex solution (PER). The ability of myometrial strips to contract, histology by light and electron microscopy as well as tissue concentrations of glutathione, ATP and protein were used as parameters to detect cold ischaemic injuries. RESULTS: Contractile ability and response to prostaglandin F2{alpha} (PGF2{alpha}) was better preserved after 6 h cold ischaemia in UW and PER in comparison with the other groups. Histological examination did not reveal any major changes after 6 and 24 h cold ischaemic storage in UW and PER solutions, while specimens stored in RIN for 24 h displayed degenerative changes on the electron microscopy level. UW and PER preserved ATP concentrations significantly better than RIN. Myometrium stored in UW contained more total glutathione but also a larger proportion of oxidized glutathione than specimens stored in RIN and PER. Protein concentrations did not change with storage time in any of the solutions. CONCLUSIONS: The results show that human uterine myometrial tissue is resistant towards cold ischaemia for at least 6 h if stored in UW and PER solutions.

Key words: ATP/contractility/cold ischaemic storage/glutathione/human uterine transplantation


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