Hum. Reprod. Advance Access originally published online on June 15, 2005
Human Reproduction 2005 20(10):2769-2775; doi:10.1093/humrep/dei118
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Andrology |
World Health Organization grade ‘a’ motility and zona-binding test accurately predict IVF outcome for mild male factor and unexplained infertilities
1 Service d’Histologie-Embryologie-Cytogénétique, Laboratoire de Biologie de la Reproduction,2 Service de Médecine de la Reproduction, Hôpital Jean Verdier, Assistance Publique – Hôpitaux de Paris, 93140 Bondy and 3 Service de Biostatistique et d’Informatique Médicale, CHU Saint-Louis, Assistance Publique – Hôpitaux de Paris, 75475 Paris cedex 10, France
4 To whom correspondence should be addressed at: Service d’Histologie-Embryologie-Cytogénétique-Laboratoire de Biologie de la Reproduction, Hôpital Jean Verdier, 93140 Bondy, France. E-mail: jean-philippe.wolf{at}jvr.ap-hop-paris.fr
BACKGROUND: The aim of this study was to determine the pronostic value of a sperm–zona pellucida (ZP) binding assay, combined with World Health Organization (WHO) grade ‘a’ sperm motility on the day of the IVF attempt, to predict sperm fertilizing ability in unexplained and moderate male factor infertilities. METHODS: In total, 84 couples (64 unexplained infertility; 20 male factor) underwent both a sperm–ZP binding assay and an IVF attempt, irrespective of the test’s result. The test was negative when grade ‘a’ motility was #5% and/or the ZP binding index was <0.7. Fertilization and pregnancy rates were related to the test’s results. RESULTS: Thirty-one patients had a negative test (group N) and 53 a positive test (group P). A difference was observed concerning the fertilization rate [median (range): 0 (0–75%) versus 50 (0–100%); P = 0.0001] and the number of cycles with fertilization rate <20% (65 versus 23%; P = 0.0002) between groups N and P respectively. In the group of unexplained and male factor infertilities, the test showed a sensitivity of 83 and 60%, specificity of 50 and 90%, positive predictive value of 76 and 86%, and negative predictive value of 61 and 69% respectively. CONCLUSION: Sperm–ZP binding test, combined with WHO grade ‘a’ motility assessment, is relevant to prevent IVF fertilization failures in unexplained infertility and, most particularly, in moderate male factor infertility.
Key words: IVF/fertilization rate/pregnancy rate/sperm–zona pellucida binding assay/sperm motility
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  D. Y. Liu, M. L. Liu, C. Garrett, and H.W. G. Baker Comparison of the frequency of defective sperm-zona pellucida (ZP) binding and the ZP-induced acrosome reaction between subfertile men with normal and abnormal semen Hum. Reprod., July 1, 2007; 22(7): 1878 - 1884. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. L.H. Evers WHO grade 'a' sperm motility and zona pellucida-binding test predict IVF outcome Hum. Reprod., January 1, 2007; 22(1): 311 - 311. [Full Text] [PDF]
|
|
|
|
|
|
C. Sifer, C. Poncelet, R. Porcher, and J.P. Wolf Reply: WHO grade 'a' sperm motility and zona pellucida-binding test predict IVF outcome Hum. Reprod., January 1, 2007; 22(1): 311 - 312. [Full Text] [PDF]
|
|
|
|
|
|
H. Ye, G.-n. Huang, Y. Gao, and D. Y. Liu Relationship between human sperm-hyaluronan binding assay and fertilization rate in conventional in vitro fertilization Hum. Reprod., June 1, 2006; 21(6): 1545 - 1550. [Abstract] [Full Text] [PDF]
|
|