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Hum. Reprod. Advance Access originally published online on December 2, 2004
Human Reproduction 2005 20(2):443-447; doi:10.1093/humrep/deh549
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Human Reproduction vol. 20 no. 2 © European Society of Human Reproduction and Embryology 2004; all rights reserved

Sperm retrieval techniques in rats with suppressed spermatogenesis by experimental cryptorchidism

Lia Mara Rossi1,6, Luís A.V. Pereira2, Luciana de Santis2, Fábio F. Pasqualotto3, Assumpto Iaconelli, Jr4, Valdemar Ortiz5 and Edson Borges, Jr4

1 Medical School at Jundiaí, and Fertility Center, 2 Universidade Estadual de Campinas, 4 Fertility Center and 5 Department of Urology, Universidade Federal São Paulo, SP and 3 Department of Embriology, Universidade de Caxias do Sul, RS, Brazil

6 To whom correspondence should be adressed at: Fertility Center for Assisted Fertilization, Avenida Brigadeiro Luís Antônio, 4.251, São Paulo, SP, Brazil. Email: lia{at}fertility.com.br

OBJECTIVE: Our aim was to assess the suppression of spermatogenesis and sperm retrieval rate after testicular sperm extraction (TESE) or testicular sperm aspiration (TESA) in adult rats with surgically induced cryptorchidism. METHODS: Adult rats were submitted to TESE and TESA procedures after 15 days of induced cryptorchidism. After spermatozoa retrieval, the testicles were extracted, weighed and a morphological analysis by conventional light microscopy was done. The numbers of spermatozoa retrieved in both TESA and TESE were rated and compared. RESULTS: Histological analysis of the testicles revealed Sertoli cell-only syndrome in 60% of the testicles, and maturation arrest in the remaining cryptorchid testicles. Significant differences were seen in the number of spermatozoa retrieved (P < 0.05) between cryptorchidic and control rats. When sperm retrieval techniques were compared, no differences were detected in the number of spermatozoa obtained (P > 0.05). CONCLUSIONS: It seems that a 15 day period of cryptorchidism is enough to induce spermatogenesis disorders. No differences were detected in the number of spermatozoa retrieved in the right or left testicles, irrespective of the testicular pole. Furthermore, and even more importantly, no differences in the retrieval rate were seen between the two techniques.

Key words: aspiration/cryptorchidism/extraction/sperm/testicle


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