Hum. Reprod. Advance Access originally published online on January 7, 2005
Human Reproduction 2005 20(4):872-880; doi:10.1093/humrep/deh717
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Lefty is expressed in mouse endometrium in estrous cycle and peri-implantation period
1 Department of Obstetrics and Gynecology, Stony Brook University, Stony Brook, NY and 2 Department of Biological Sciences, University of Delaware, Newark, DE 19716, USA
3 To whom correspondence should be addressed at: Frontiers in Bioscience, P.O.Box 160, Searingtown, NY 11507, USA. Email: tabibzadeh{at}bioscience.org
BACKGROUND: Reproductive tissues are unique structures that exhibit cyclic stromal remodelling during menstrual cycles in humans. Ebaf/lefty participates in tissue remodelling of human endometrium by induction of matrix metalloproteases (MMP).METHODS: We describe the temporal expression and spatial distribution of lefty and tissue remodelling events in mouse endometrium. RT–PCR and real-time PCR were used to identify mRNA expression and western blots to analyse Lefty protein. Immunolocalization was performed with specific antibodies and horseradish peroxidase staining. RESULTS: Lefty was expressed in endometrium throughout the estrous cycle. Expression of MMP (MMP-2, -3, -7 and -14) was higher at estrus, metestrus and/or diestrus while collagen content of endometrium decreased in these phases. During pregnancy, lefty levels were higher on days 3–5 and were minimal by day 9. Similarly, expression of endometrial MMP was higher on days 3 and 5 of pregnancy and was low on day 9. During pregnancy, loss of collagen was initiated on day 3, persisted to day 5, and led to a significantly reduced collagen on day 9. Immunoreactive lefty decorated basal laminae, and was associated with extracellular matrix in stroma. CONCLUSIONS: Regulated expression and spatial distribution of lefty in mouse endometrium confines its biological impact on tissues that undergo remodelling during estrous cycle and pregnancy.
Key words: Ebaf/endometrium/estrous cycle/lefty/mouse
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  S. Tabibzadeh and A. Hemmati-Brivanlou Lefty at the Crossroads of "Stemness" and Differentiative Events Stem Cells, September 1, 2006; 24(9): 1998 - 2006. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. L Jones, C. Stoikos, J. K Findlay, and L. A Salamonsen TGF-{beta} superfamily expression and actions in the endometrium and placenta. Reproduction, August 1, 2006; 132(2): 217 - 232. [Abstract] [Full Text] [PDF]
|
|