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Hum. Reprod. Advance Access originally published online on February 10, 2005
Human Reproduction 2005 20(5):1228-1234; doi:10.1093/humrep/deh773
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© The Author 2005. Published by Oxford University Press on behalf of the European Society of Human Reproduction and Embryology. All rights reserved. For Permissions, please email: journals.permissions{at}oupjournals.org

Increased cystic fibrosis transmembrane conductance regulator (CFTR) expression in the human hydrosalpinx

Louis Chukwuemeka Ajonuma1, Ernest Hung Yu Ng2, Pak Ham Chow4, Cathy Yui Hung4, Lai Ling Tsang1, Annie Nga Yin Cheung3, Christine Brito-Jones5, Ingrid Hung Lok5, Christopher J.Haines5 and Hsiao Chang Chan1,6

1 Epithelial Cell Biology Research Center, Department of Physiology, Faculty of Medicine, Chinese University of Hong Kong, Shatin, NT, Hong Kong, 2 Department of Obstetrics & Gynecology, 3 Department of Pathology, Faculty of Medicine, The University of Hong Kong, and 4 Department of Anatomy and 5 Department of Obstetrics & Gynecology, Faculty of Medicine, The Chinese University of Hong Kong, SAR, China

6 To whom correspondence should be addressed. Email: hsiaocchan{at}cuhk.edu.hk

BACKGROUND: Hydrosalpinx (HSP), characterized by abnormal fluid accumulation in the Fallopian tube, is one of the main causes of infertility in women; however, the mechanism underlying the formation of hydrosalpinx fluid (HF) remains elusive. The present study investigated the possible involvement of cystic fibrosis transmembrane conductance regulator (CFTR), a cAMP-dependent chloride channel, in the pathogenesis of hydrosalpinx. METHODS: Masson's trichrome staining was used to characterize epithelial transformation in human HSP; RT–PCR, immunohistochemistry and immunofluorescence staining were used for CFTR expression and localization. RESULTS: Masson's trichrome staining showed areas of epithelial transformation, focally attenuated and pseudostratified. Immunostaining showed enhanced CFTR immunoreactivity in the focally attenuated and pseudostratified areas of HSP epithelium. RT–PCR revealed that CFTR expression in HSP was significantly greater than that in normal Fallopian tubes. CONCLUSIONS: These results indicate that HSP epithelium undergoes epithelial transformation with elevated CFTR expression, which may lead to increased transepithelial electrolyte and fluid secretion resulting in HF formation. The present findings may lead to the development of new treatment strategies for infertile patients with HSP.

Key words: CFTR/female reproductive tract/hydrosalpinx/hydrosalpinx fluid/infertility


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