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Hum. Reprod. Advance Access originally published online on April 14, 2005
Human Reproduction 2005 20(7):1933-1937; doi:10.1093/humrep/deh899
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© The Author 2005. Published by Oxford University Press on behalf of the European Society of Human Reproduction and Embryology. All rights reserved. For Permissions, please email: journals.permissions{at}oupjournals.org

A trial to restore defective human sperm centrosomal function

Soichi Nakamura1, Yukihiro Terada1,4, Vanesa Y. Rawe2, Shigeki Uehara3, Yuki Morito1, Tomoko Yoshimoto1, Masahito Tachibana1, Takashi Murakami1, Nobuo Yaegashi1 and Kunihiro Okamura1

1 Department of Obstetrics and Gynecology, Tohoku University School of Medicine, 1–1 Seiryo-machi, Aoba-ku, Sendai, Miyagi 980-8574, Japan, 2 Pittsburgh Development Center, Magee–Woman's Research Institute, Departments of Obstetrics, Gynecology and Reproductive Sciences, and Cell Biology and Physiology, University of Pittsburgh School of Medicine, Pittsburgh, PA 15213, USA and 3 Department of Obstetrics and Gynecology, Tohoku Kosai Hospital, 2-3-11 Kokubun-chou, Aoba-ku, Sendai, Miyagi 980-0803, Japan

4 To whom correspondence should be addressed. Email: terada{at}mail.tains.tohoku.ac.jp

BACKGROUND: In human fertilization, sperm centrosome function is essential for male and female pronuclear movement and fusion. In this study, we investigated the possibility of restoring human sperm centrosomal function in sperm exhibiting abnormalities in microtubule organization. METHODS: Semen was obtained from both a fertile donor and a patient with dysplasia of the fibrous sheath (DFS). Following heterologous ICSI using human sperm, we examined microtubules and chromatin configuration in bovine oocytes. Sperm were treated with dithiothreitol (DTT) prior to ICSI, while the oocytes were treated with the cytoskeletal stabilizer paclitaxel after ICSI. RESULTS: The combination of DTT and paclitaxel treatment induced microtubule organization in dead sperm from the fertile donor following heterologous ICSI. This treatment, however, was not effective for DFS sperm. In addition, expression of centrin, a protein functioning within the sperm centrosome, was reduced in DFS sperm from that of the normal levels observed in fertile donor sperm. CONCLUSION: These results indicate that sperm centrosomal function could be induced by the treatment of sperm with DTT before ICSI and of oocytes with paclitaxel after ICSI. DFS sperm are likely to exhibit such severe dysfunction of sperm centrosome that cannot be compensated for by this treatment; therefore, this method may be a practical way to discern the degree of sperm centrosomal dysfunction.

Key words: cytoskeleton/drug/fertilization/infertility/sperm centrosome


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[Abstract] [Full Text] [PDF]


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