Skip Navigation


Hum. Reprod. Advance Access originally published online on April 14, 2005
Human Reproduction 2005 20(8):2092-2096; doi:10.1093/humrep/dei012
This Article
Right arrow Full Text Freely available
Right arrow FREE Full Text (PDF ) Freely available
Right arrow All Versions of this Article:
20/8/2092    most recent
dei012v1
Right arrow Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Hirata, T.
Right arrow Articles by Taketani, Y.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Hirata, T.
Right arrow Articles by Taketani, Y.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

© The Author 2005. Published by Oxford University Press on behalf of the European Society of Human Reproduction and Embryology. All rights reserved. For Permissions, please email: journals.permissions{at}oupjournals.org

Development of an experimental model of endometriosis using mice that ubiquitously express green fluorescent protein

Tetsuya Hirata, Yutaka Osuga1, Osamu Yoshino, Yasushi Hirota, Miyuki Harada, Yuri Takemura, Chieko Morimoto, Kaori Koga, Tetsu Yano, Osamu Tsutsumi and Yuji Taketani

Department of Obstetrics and Gynecology, Faculty of Medicine, University of Tokyo, 7-3-1, Hongo, Bunkyo-ku, Tokyo, 113-8655, Japan

1 To whom correspondence should be addressed. Email: yutakaos-tky{at}umin.ac.jp

BACKGROUND: Aiming at improving an animal model of endometriosis, we developed a homologous mouse model using ‘green mice’ that ubiquitously express green fluorescent protein. METHODS: Endometrial fragment obtained from estradiol (E2)-supplemented ovariectomized ‘green mice’ was minced and injected into the peritoneal cavity of ovariectomized wild-type mice. The recipient wild mice were raised with or without E2 supplementation for 2 weeks, and then were euthanized. Endometriotic lesions that developed in the abdomen were examined both macroscopically and microscopically under fluorescence, and weight of the lesions was measured. RESULTS: The endometriotic lesions were more clearly detected under fluorescence imaging than by conventional macroscopic examination. Histologically, endometriotic lesions deriving from ‘green mice’ were sharply distinguished from surrounding host tissues under fluorescence microscopy. More lesions developed in E2-supplemented than control recipient mice. The measured fluorescence intensity of endometriotic lesions showed significant positive correlation with their weight (R=0.844, P<0.0001), and was significantly higher in E2-supplemented mice than in vehicle-supplemented mice (P=0.0062). CONCLUSION: The present endometriosis model using ‘green mice’ would be useful for expeditious identification and quantitative evaluation of endometriotic lesions.

Key words: animal model/endometriosis/estrogen/green fluorescent protein/histology


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Hum ReprodHome page
N.M. Nowak, O.M. Fischer, T.C. Gust, U. Fuhrmann, U.-F. Habenicht, and A. Schmidt
Intraperitoneal inflammation decreases endometriosis in a mouse model
Hum. Reprod., August 16, 2008; (2008) den189v2.
[Abstract] [Full Text] [PDF]

Home page
 Hum Reprod UpdateHome page
R. Grummer
Animal models in endometriosis research
Hum. Reprod. Update, September 1, 2006; 12(5): 641 - 649.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Pathol.Home page
C. M. Becker, R. D. Wright, R. Satchi-Fainaro, T. Funakoshi, J. Folkman, A. L. Kung, and R. J. D'Amato
A Novel Noninvasive Model of Endometriosis for Monitoring the Efficacy of Antiangiogenic Therapy
Am. J. Pathol., June 1, 2006; 168(6): 2074 - 2084.
[Abstract] [Full Text] [PDF]

Home page
 Hum ReprodHome page
S. Defrere, A. Van Langendonckt, R. G. Ramos, M. Jouret, M. Mettlen, and J. Donnez
Quantification of endometriotic lesions in a murine model by fluorimetric and morphometric analyses
Hum. Reprod., March 1, 2006; 21(3): 810 - 817.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
Y.-J. Lin, M.-D. Lai, H.-Y. Lei, and L.-Y. C. Wing
Neutrophils and Macrophages Promote Angiogenesis in the Early Stage of Endometriosis in a Mouse Model
Endocrinology, March 1, 2006; 147(3): 1278 - 1286.
[Abstract] [Full Text] [PDF]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.