Skip Navigation


Hum. Reprod. Advance Access originally published online on May 26, 2005
Human Reproduction 2005 20(9):2376-2382; doi:10.1093/humrep/dei096
This Article
Right arrow Full Text Freely available
Right arrow FREE Full Text (PDF ) Freely available
Right arrow All Versions of this Article:
20/9/2376    most recent
dei096v1
Right arrow Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (7)
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Kanatsu-Shinohara, M.
Right arrow Articles by Shinohara, T.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Kanatsu-Shinohara, M.
Right arrow Articles by Shinohara, T.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

© The Author 2005. Published by Oxford University Press on behalf of the European Society of Human Reproduction and Embryology. All rights reserved. For Permissions, please email: journals.permissions{at}oupjournals.org

Germline niche transplantation restores fertility in infertile mice

M. Kanatsu-Shinohara1,2, H. Miki3, K. Inoue3, N. Ogonuki3, S. Toyokuni4, A. Ogura3 and T. Shinohara2,5

1 Horizontal Medical Research Organization and 4 Department of Pathology and Biology of Diseases, Graduate School of Medicine, Kyoto University, Kyoto 606-8501, 2 Department of Molecular Genetics, Graduate School of Medicine, Kyoto University, Kyoto 606-8507 and 3 The Institute of Physical and Chemical Research (RIKEN), Bioresource Center, Ibaraki 305-0074, Japan

5 To whom correspondence should be addressed. Email: tshinoha{at}virus.kyoto-u.ac.jp

BACKGROUND: Stem cells interact closely with their microenvironment or niche, and abnormalities in niche compromise the self-renewing tissue. In testis, for example, Sertoli cells interact with germ cells, and defects in Sertoli cells compromises spermatogenesis, leading to male infertility. However, it has not been possible to restore spermatogenesis from endogenous stem cells in infertile testis with environmental defects METHODS AND RESULTS: When healthy Sertoli cells from infertile white spotting (W) mouse were transplanted into the seminiferous tubules of infertile Steel (Sl) mouse testis that had defective Sertoli cells, spermatogenesis occurred from Sl stem cells in the recipient testis. On average, 1.1% of the recipient tubules showed spermatogenesis. Furthermore, in a microinsemination experiment with germ cells that developed in the testis, we obtained four normal offspring from 114 successfully injected oocytes. CONCLUSIONS: This study demonstrates that defects in male germline microenvironment can be corrected by Sertoli cell transplantation. Although further improvements are required to enhance the low efficiency of spermatogenesis, the ability to correct environmental defect by niche transplantation has important implications in developing new strategies for treating incurable disorders in self-renewing tissues.

Key words: infertility/niche/Sertoli cells/spermatogenesis/transplantation


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Hum Mol GenetHome page
N. Geijsen and D. L. Jones
Seminal discoveries in regenerative medicine: contributions of the male germ line to understanding pluripotency
Hum. Mol. Genet., April 15, 2008; 17(R1): R16 - R22.
[Abstract] [Full Text] [PDF]

Home page
 Reproductive SciencesHome page
C. E. Gargett
Review Article: Stem Cells in Human Reproduction
Reproductive Sciences, July 1, 2007; 14(5): 405 - 424.
[Abstract] [PDF]

Home page
 Am. J. Pathol.Home page
Y. Lue, K. Erkkila, P. Y. Liu, K. Ma, C. Wang, A. S. Hikim, and R. S. Swerdloff
Fate of Bone Marrow Stem Cells Transplanted into the Testis: Potential Implication for Men with Testicular Failure
Am. J. Pathol., March 1, 2007; 170(3): 899 - 908.
[Abstract] [Full Text] [PDF]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.