Skip Navigation


Hum. Reprod. Advance Access originally published online on June 2, 2005
Human Reproduction 2005 20(9):2383-2390; doi:10.1093/humrep/dei078
This Article
Right arrow Full Text Freely available
Right arrow FREE Full Text (PDF ) Freely available
Right arrow All Versions of this Article:
20/9/2383    most recent
dei078v1
Right arrow Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (5)
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Younglai, E.V.
Right arrow Articles by Kwan, C.-Y.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Younglai, E.V.
Right arrow Articles by Kwan, C.-Y.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

© The Author 2005. Published by Oxford University Press on behalf of the European Society of Human Reproduction and Embryology. All rights reserved. For Permissions, please email: journals.permissions{at}oupjournals.org

Non-genomic action of estradiol and progesterone on cytosolic calcium concentrations in primary cultures of human granulosa-lutein cells

E.V. Younglai1,3, Y.J. Wu1, T.K. Kwan2 and C.-Y. Kwan2

1 Department of Obstetrics and Gynecology, Reproductive Biology Division and 2 Department of Medicine, McMaster University, Health Sciences Centre, Hamilton, Ontario, Canada L8N 3Z5

3 To whom correspondence should be addressed at: Department of Obstetrics and Gynecology, McMaster University Medical Centre, 1200 Main Street West, Hamilton, Ontario, Canada L8N 3Z5. Email: younglai{at}mcmaster.ca

BACKGROUND: The present study examined whether the sex steroids, estradiol and progesterone, could alter cytoplasmic calcium concentrations ([Ca2+]cyt) in human granulosa-lutein cells. METHODS: Human granulosa cells were obtained at the time of oocyte retrieval for IVF and cultured for 3–7 days. Cells were loaded with Fura-2 AM and changes in [Ca2+]cyt of single cells were studied using a dynamic digital Ca2+ imaging system. RESULTS: Both estradiol and progesterone stimulated elevations of [Ca2+]cyt in Ca2+-containing medium within seconds of exposure of the granulosa-lutein cells to the steroid, but only estradiol caused an increase in [Ca2+]cyt in Ca2+-free medium. Both ICI-182780 and RU 486 stimulated [Ca2+]cyt increases and inhibited the effects of estradiol and progesterone, respectively. Tamoxifen also induced transient increases in [Ca2+]cyt concentrations but inhibited the effects of both estradiol and progesterone. The inhibitory effects of tamoxifen, ICI-182780 and RU 4486 on [Ca2+]cyt responses to estradiol and progesterone could be reversed with higher concentrations of estradiol and progesterone, respectively. The [Ca2+]cyt effects induced with tamoxifen could not be eliminated by prior treatment with RU 486 or ICI-182780. CONCLUSION: These results provide strong evidence that both estradiol and progesterone as well as the steroid antagonists, tamoxifen, RU 486 and ICI-182780, can act on human granulosa-lutein cells through a non-genomic mechanism.

Key words: calcium response/estradiol/granulosa cells/progesterone


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 ReproductionHome page
Y. Wu, W. G Foster, and E. V Younglai
Rapid effects of pesticides on human granulosa-lutein cells
Reproduction, February 1, 2006; 131(2): 299 - 310.
[Abstract] [Full Text] [PDF]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.