Skip Navigation


Hum. Reprod. Advance Access originally published online on June 9, 2005
Human Reproduction 2005 20(9):2502-2508; doi:10.1093/humrep/dei099
This Article
Right arrow Full Text Freely available
Right arrow FREE Full Text (PDF ) Freely available
Right arrow All Versions of this Article:
20/9/2502    most recent
dei099v1
Right arrow Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Samuel Kim, S.
Right arrow Articles by Lee, H. H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Samuel Kim, S.
Right arrow Articles by Lee, H. H.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

© The Author 2005. Published by Oxford University Press on behalf of the European Society of Human Reproduction and Embryology. All rights reserved. For Permissions, please email: journals.permissions{at}oupjournals.org

Assessment of the integrity of human oocytes retrieved from cryopreserved ovarian tissue after xenotransplantation

S. Samuel Kim1,3, Hee Gyu Kang1, Nam Hyung Kim2, Hoi Chang Lee1 and Hyang Heun Lee1

1 Department of OB/GYN, Eulji University School of Medicine, Seoul and 2 Department of Animal Sciences, Chungbuk National University, Chong Ju, Korea

3 To whom correspondence should be addressed at: Center for Reproductive Medicine, Department of Obstetrics and Gynecology, Cedars–Sinai Medical Center, Los Angeles, CA 90048, USA. Email: medssk{at}attglobal.net

BACKGROUND: Previous studies showed that immature oocytes stored in ovarian tissue could develop to the mature stage after transplantation. However, the quality and competency of the oocytes developed in xenografted ovarian tissue have never been investigated. As a pilot study to investigate this uncharted issue, we evaluated microtubule organization and chromatin configuration of human oocytes harvested from xenografted frozen–thawed ovarian tissue. METHODS: Frozen–thawed human ovarian tissue was transplanted into severe combined immunodeficient mice. All animals were stimulated with gonadotrophin from 20 weeks after transplantation. Grafts were recovered 36 h after hCG administration. The oocytes were retrieved from the antral follicles (>2 mm diameter), cultured in vitro, stained for microtubule and chromatin localization. RESULTS: Five oocytes from 21 female mice and seven oocytes from nine male mice were retrieved. Immunocytochemical examinations of these oocytes after in vitro maturation revealed only two developed to the metaphase II stage. Most oocytes were between prophase and metaphase with abnormal microtubule organization and chromatin configuration. CONCLUSIONS: Immature oocytes in stored human ovarian tissue can grow to maturity in host animals after xenotransplantation. Retrieval of oocytes from the xenograft can be carried out and is reproducible. However, many oocytes, grown in host animals and further matured in vitro, showed aberrant microtubule organization and chromatin patterns.

Key words: cryopreservation/microtubule/oocyte/ovarian tissue/transplantation


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Hum Reprod UpdateHome page
I. Demeestere, P. Simon, S. Emiliani, A. Delbaere, and Y. Englert
Orthotopic and heterotopic ovarian tissue transplantation
Hum. Reprod. Update, November 1, 2009; 15(6): 649 - 665.
[Abstract] [Full Text] [PDF]

Home page
 Hum ReprodHome page
R. Soleimani, J. Van der Elst, E. Heytens, R. Van den Broecke, J. Gerris, M. Dhont, C. Cuvelier, and P. De Sutter
Back muscle as a promising site for ovarian tissue transplantation, an animal model
Hum. Reprod., March 1, 2008; 23(3): 619 - 626.
[Abstract] [Full Text] [PDF]

Home page
 Hum Reprod UpdateHome page
J. Donnez, B. Martinez-Madrid, P. Jadoul, A. Van Langendonckt, D. Demylle, and M.-M. Dolmans
Ovarian tissue cryopreservation and transplantation: a review
Hum. Reprod. Update, September 1, 2006; 12(5): 519 - 535.
[Abstract] [Full Text] [PDF]

Home page
 Hum ReprodHome page
I. Demeestere, P. Simon, F. Buxant, V. Robin, S. A. Fernandez, J. Centner, A. Delbaere, and Y. Englert
Ovarian function and spontaneous pregnancy after combined heterotopic and orthotopic cryopreserved ovarian tissue transplantation in a patient previously treated with bone marrow transplantation: Case Report
Hum. Reprod., August 1, 2006; 21(8): 2010 - 2014.
[Abstract] [Full Text] [PDF]

Home page
 The OncologistHome page
M. Sonmezer and K. Oktay
Fertility preservation in young women undergoing breast cancer therapy.
Oncologist, May 1, 2006; 11(5): 422 - 434.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.