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Hum. Reprod. Advance Access originally published online on September 9, 2005
Human Reproduction 2006 21(1):138-144; doi:10.1093/humrep/dei285
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© The Author 2005. Published by Oxford University Press on behalf of the European Society of Human Reproduction and Embryology. All rights reserved. For Permissions, please email: journals.permissions@oupjournals.org

Decreased mRNA transcripts of M-phase promoting factor and its regulators in the testes of infertile men

Yung Ming Lin1, Yen Ni Teng2, Chia Ling Chung3, Wan Ching Tsai1, Ying Hung Lin4, Johnny Shinn Nan Lin1 and Pao Lin Kuo3,5

Y.M.Lin and Y.N.Teng contributed equally to this work. Departments of 1 Urology, 3 Obstetrics & Gynecology, and 4 Institute of Basic Medical Science, National Cheng Kung University, College of Medicine, Tainan, and 2 Department of Early Childhood Education and Nursery, Chia Nan University of Pharmacy and Science, Tainan, Taiwan

5 To whom correspondence should be addressed at: Division of Genetics, Department of Obstetrics and Gynecology, National Cheng Kung University Hospital, 138 Sheng Li Road, Tainan, Taiwan 704. E-mail: paolink{at}mail.ncku.edu.tw

BACKGROUND: M-phase promoting factor (MPF), which is comprised of Cyclin B and a catalytic subunit, Cdc2, is a key enzyme required for cells to enter M phase in both mitosis and meiosis. MPF activity is controlled by the stimulatory dephosphorylation of the Cdc25 family and the inhibitory phosphorylation of Wee1. We determined the levels of mRNA transcripts of MPF and its regulators in the testes of infertile men, and evaluated the relationship between the transcript levels and patients’ testicular phenotypes and sperm retrieval results. METHODS AND RESULTS: The mRNA transcript levels of CDC2, CCNB1, CCNB2, CDC25A, CDC25B, CDC25C and WEE1 in the testes of 37 azoospermic patients were examined by quantitative real-time polymerase chain reaction. Significant decreases in CDC2, CCNB1, CCNB2, CDC25A, CDC25C and WEE1 mRNA transcript levels were detected in patients with spermatogenic failure. CDC2 mRNA transcript levels correlated significantly with those of CCNB1 and CCNB2 mRNA. Significantly higher CDC2, CCNB1, CCNB2, CDC25C and WEE1 mRNA transcript levels were detected in 18 patients with successful sperm retrieval than in 11 patients with failed sperm retrieval. CONCLUSIONS: We suggest that the decreased mRNA transcripts of MPF and its regulators play important roles in human spermatogenesis.

Key words: male infertility/MPF/testis


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Y. S. Cheng, P. L. Kuo, Y. N. Teng, T. Y. Kuo, C. L. Chung, Y. H. Lin, R. W. Liao, J. S. N. Lin, and Y. M. Lin
Association of spermatogenic failure with decreased CDC25A expression in infertile men
Hum. Reprod., September 1, 2006; 21(9): 2346 - 2352.
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