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Hum. Reprod. Advance Access originally published online on August 7, 2006
Human Reproduction 2006 21(11):2960-2968; doi:10.1093/humrep/del257
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© The Author 2006. Published by Oxford University Press on behalf of the European Society of Human Reproduction and Embryology. All rights reserved. For Permissions, please email: journals.permissions@oxfordjournals.org

Peptide and protein profiles in serum and follicular fluid of women undergoing IVF

Florian J. Schweigert1,4, Beate Gericke1, Wiebke Wolfram1, Udo Kaisers2 and Joachim W. Dudenhausen3

1 Institute of Nutritional Science, University of Potsdam, Potsdam-Rehbrücke, Germany 2 Department of Anesthesiology and Intensive Care Medicine and 3 Department of Obstetrics, University Medicine Berlin, Berlin, Germany

4 To whom correspondence should be addressed at: Institute of Nutritional Science, University of Potsdam, Arthur-Scheunert-Allee 114-116, D-14558 Potsdam-Rehbrücke, Germany. E-mail: schweigert{at}nutriproteomics.de

BACKGROUND: Proteins and peptides in human follicular fluid originate from plasma or are produced by follicular structures. Compositional changes reflect oocyte maturation and can be used as diagnostic markers. The aim of the study was to determine protein and peptide profiles in paired serum and follicular fluid samples from women undergoing IVF. METHODS: Surface-enhanced laser desorption and ionization–time of flight–mass spectrometry (SELDI-TOF-MS) was used to obtain characteristic protein pattern. RESULTS: One hundred and eighty-six individual MS signals were obtained from a combination of enrichment on strong anion exchanger (110), weak cation exchanger (52) and normal phase surfaces (24). On the basis of molecular masses, isoelectric points and immunoreactivety, four signals were identified as haptoglobin ({alpha}1- and {alpha}2-chain), haptoglobin 1 and transthyretin (TTR). Immunological and MS characteristics of the TTR : retinol-binding protein (RBP) transport complex revealed no microheterogeneity differences between serum and follicular fluid. Discriminatory patterns arising from decision-tree-based classification and regression analysis distinguished between serum and follicular fluid with a sensitivity and specificity of 100%. CONCLUSIONS: Quantitative and qualitative differences indicate selective transport processes rather than mere filtration across the blood-follicle barrier. Identified proteins as well as characteristic peptide and/or protein signatures might emerge as potential candidates for diagnostic markers of follicle and/or oocyte maturation and thus oocyte quality.

Key words: human follicular fluid/peptide/protein/proteome/serum


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