Skip Navigation


Hum. Reprod. Advance Access originally published online on December 8, 2005
Human Reproduction 2006 21(3):685-693; doi:10.1093/humrep/dei401
This Article
Right arrow Full Text Freely available
Right arrow FREE Full Text (PDF ) Freely available
Right arrow All Versions of this Article:
21/3/685    most recent
dei401v1
Right arrow Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (14)
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Brugnon, F.
Right arrow Articles by Van Steirteghem, A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Brugnon, F.
Right arrow Articles by Van Steirteghem, A.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

© The Author 2005. Published by Oxford University Press on behalf of the European Society of Human Reproduction and Embryology. All rights reserved. For Permissions, please email: journals.permissions@oxfordjournals.org

Study of two markers of apoptosis and meiotic segregation in ejaculated sperm of chromosomal translocation carrier patients

F. Brugnon1,4,5, E. Van Assche3, G. Verheyen3, B. Sion1,4, D. Boucher4, J.L. Pouly2, L. Janny1, P. Devroey3, I. Liebaers3 and A. Van Steirteghem3

1 Biologie de la Reproduction, CECOS, 2 Département de gynécologie obstétrique et reproduction humaine and 3 Research Centre Reproductive and Genetics, Vrije Universiteit Brussel, Laarbeeklaan 101, B-1090, Belgium and 4 EA 975, Biologie de la Reproduction, Faculté de Médecine, BP 38, 63000 Clermont-Ferrand, France

5 To whom correspondence should be addressed: fbrugnon{at}chu-clermontferrand.fr

BACKGROUND: To try to explain the infertility of chromosomal translocation carrier patients, we compared the expression of two markers of apoptosis in the sperm of patients and of fertile donors, and we studied the meiotic segregation in the ejaculated sperm of these translocation carriers. METHODS: Twenty semen samples of translocation carriers, [reciprocal (n = 14) and Robertsonian translocations (n = 6)], were compared with the semen samples of donors (n = 20). Different tests were applied: annexin V binding assay; terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end labelling (TUNEL); and fluorescence in situ hybridization (FISH). RESULTS: The annexin V binding assay in sperm of patients with chromosomal translocation (n = 17) showed a significantly increased proportion of sperm with externalized phosphatidylserine (PS) than in the control group (n = 20, P ≤ 0.05). The rates of DNA fragmentation investigated by TUNEL reaction were higher in samples of translocation carriers (n = 14) than in donors (n = 20, P < 0.0001). The measures by FISH technique showed that the proportions of balanced or normal gametes were predominant in the reciprocal translocation group (alternate: n = 7; from 33.0 to 58.8%; adjacent I: n = 7; from 4.6 to 43.8%) and in the Robertsonian translocation group (normal: n = 5; from 76.0 to 88.5%). CONCLUSIONS: Our data show a predominant proportion of balanced gametes in sperm of chromosomal translocation carrier patients. Moreover, PS externalization and DNA fragmentation rates are significantly higher in ejaculated sperm of these patients than in donor sperm. These tests could be used to predict the outcome of ICSI for these patients.

Key words: apoptosis/chromosomal translocation/DNA fragmentation/meiotic segregation/phosphatidylserine externalization


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Hum Reprod UpdateHome page
R.H. Martin
Cytogenetic determinants of male fertility
Hum. Reprod. Update, June 4, 2008; (2008) dmn017v1.
[Abstract] [Full Text] [PDF]

Home page
 Hum ReprodHome page
A. Perrin, N. Douet-Guilbert, M.J. Le Bris, G. Keromnes, M.L. Langlois, P. Barriere, J. Amice, V. Amice, M. De Braekeleer, and F. Morel
Segregation of chromosomes in sperm of a t(X;18)(q11;p11.1) carrier inherited from his mother: Case Report
Hum. Reprod., January 1, 2008; 23(1): 227 - 230.
[Abstract] [Full Text] [PDF]

Home page
 Hum ReprodHome page
E. Anton, F. Vidal, and J. Blanco
Role of sperm FISH studies in the genetic reproductive advice of structural reorganization carriers
Hum. Reprod., August 1, 2007; 22(8): 2088 - 2092.
[Abstract] [Full Text] [PDF]

Home page
 Hum ReprodHome page
A. Perrin, N. Douet-Guilbert, B. Laudier, M.L. Couet, F. Guerif, D. Royere, M.J. Le Bris, M. De Braekeleer, and F. Morel
Meiotic segregation in spermatozoa of a 45,XY,-14,der(18)t(14;18)(q11;p11.3) translocation carrier: A Case Report
Hum. Reprod., March 1, 2007; 22(3): 729 - 732.
[Abstract] [Full Text] [PDF]

Home page
 Hum ReprodHome page
K. Moradkhani, J. Puechberty, S. Bhatt, J. Lespinasse, P. Vago, G. Lefort, P. Sarda, S. Hamamah, and F. Pellestor
Rare Robertsonian translocations and meiotic behaviour: sperm FISH analysis of t(13;15) and t(14;15) translocations: A Case Report
Hum. Reprod., December 1, 2006; 21(12): 3193 - 3198.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.