Skip Navigation


Hum. Reprod. Advance Access originally published online on January 20, 2006
Human Reproduction 2006 21(4):1002-1008; doi:10.1093/humrep/dei435
This Article
Right arrow Full Text Freely available
Right arrow FREE Full Text (PDF ) Freely available
Right arrow All Versions of this Article:
21/4/1002    most recent
dei435v1
Right arrow Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (8)
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Liu, D.Y.
Right arrow Articles by Baker, H.W.G.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Liu, D.Y.
Right arrow Articles by Baker, H.W.G.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

© The Author 2006. Published by Oxford University Press on behalf of the European Society of Human Reproduction and Embryology. All rights reserved. For Permissions, please email: journals.permissions@oxfordjournals.org

Tyrosine phosphorylation on capacitated human sperm tail detected by immunofluorescence correlates strongly with sperm–zona pellucida (ZP) binding but not with the ZP-induced acrosome reaction

D.Y. Liu1,5, G.N. Clarke2 and H.W.G. Baker1,3,4

1 Department of Obstetrics and Gynaecology, University of Melbourne, 2 Andrology Laboratory and 3 Reproductive Services, Royal Women’s Hospital, 4 Melbourne IVF, Melbourne, Australia

5 To whom correspondence should be addressed. E-mail: dyl{at}unimelb.edu.au

BACKGROUND: Protein tyrosine phosphorylation (TP) of human sperm is related to sperm capacitation and zona pellucida (ZP) binding. The aim of this study was to determine whether the TP of capacitated sperm is a useful marker for the ability of sperm to bind to the ZP and undergo the ZP-induced acrosome reaction (AR). METHODS: Semen samples were obtained from 115 subfertile men with sperm count ≥20 x 106/ml, motility ≥25% and variable morphology. Motile sperm (2 x 106/ml) selected by swim-up were incubated with four oocytes for 2 h, and the number of sperm bound to the ZP and the ZP-induced AR was examined. TP of sperm tail was assessed by immunofluorescence (IF) with anti-phosphotyrosine monoclonal antibody. The time course and effects of dibutyryl cyclic adenosine monophosphate (dbcAMP) and phorbol myristate acetate (PMA) on TP were also studied. RESULTS: TP was stimulated more by dbcAMP (P < 0.001) and less by PMA (P < 0.05). TP increased significantly with time of incubation of sperm. TP was not detectable on the surface of unfixed live sperm by either Dynabeads or IF. Sperm TP at 2, 4 and 20 h incubation was all significantly correlated with sperm–ZP binding but not with the ZP-induced AR. CONCLUSION: Sperm TP detected by IF correlates strongly with sperm–ZP binding capacity but not with the ZP-induced AR. This simple IF assay of TP may be a clinically useful test of sperm function that is predictive of normal sperm ZP-binding capacity.

Key words: immunofluorescence/sperm–zona binding/tyrosine phosphorylation/zona-induced acrosome reaction


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 ReproductionHome page
P. Grasa, C. Colas, M. Gallego, L. Monteagudo, T. Muino-Blanco, and J. A. Cebrian-Perez
Changes in content and localization of proteins phosphorylated at tyrosine, serine and threonine residues during ram sperm capacitation and acrosome reaction
Reproduction, April 1, 2009; 137(4): 655 - 667.
[Abstract] [Full Text] [PDF]

Home page
 Hum ReprodHome page
D.Y. Liu, M.L. Liu, and H.W.G. Baker
Enhancement of sperm-zona pellucida (ZP) binding capacity by activation of protein kinase A and C pathways in certain infertile men with defective sperm-ZP binding
Hum. Reprod., January 1, 2009; 24(1): 20 - 27.
[Abstract] [Full Text] [PDF]

Home page
 Biol. Reprod.Home page
A. Barbonetti, M.R.C. Vassallo, B. Cinque, C. Antonangelo, F. Sciarretta, R. Santucci, A. D'Angeli, S. Francavilla, and F. Francavilla
Dynamics of the Global Tyrosine Phosphorylation During Capacitation and Acquisition of the Ability to Fuse with Oocytes in Human Spermatozoa
Biol Reprod, October 1, 2008; 79(4): 649 - 656.
[Abstract] [Full Text] [PDF]

Home page
 Biol. Reprod.Home page
P.-h. Hung, M. G. Miller, S. A. Meyers, and C. A. VandeVoort
Sperm Mitochondrial Integrity Is Not Required for Hyperactivated Motility, Zona Binding, or Acrosome Reaction in the Rhesus Macaque
Biol Reprod, August 1, 2008; 79(2): 367 - 375.
[Abstract] [Full Text] [PDF]

Home page
 Biol. Reprod.Home page
Z. Zhang, X. Shen, B. H Jones, B. Xu, J. C Herr, and J. F Strauss III
Phosphorylation of Mouse Sperm Axoneme Central Apparatus Protein SPAG16L by a Testis-Specific Kinase, TSSK2
Biol Reprod, July 1, 2008; 79(1): 75 - 83.
[Abstract] [Full Text] [PDF]

Home page
 Mol Hum ReprodHome page
L.A. Mitchell, B. Nixon, and R.J. Aitken
Analysis of chaperone proteins associated with human spermatozoa during capacitation
Mol. Hum. Reprod., September 1, 2007; 13(9): 605 - 613.
[Abstract] [Full Text] [PDF]

Home page
 Hum ReprodHome page
D. Y. Liu, M. L. Liu, C. Garrett, and H.W. G. Baker
Comparison of the frequency of defective sperm-zona pellucida (ZP) binding and the ZP-induced acrosome reaction between subfertile men with normal and abnormal semen
Hum. Reprod., July 1, 2007; 22(7): 1878 - 1884.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.