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Hum. Reprod. Advance Access originally published online on March 29, 2006
Human Reproduction 2006 21(7):1705-1719; doi:10.1093/humrep/del065
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© The Author 2006. Published by Oxford University Press on behalf of the European Society of Human Reproduction and Embryology. All rights reserved. For Permissions, please email: journals.permissions@oxfordjournals.org The online version of this article has been published under an open access model. Users are entitled to use, reproduce, disseminate, or display the open access version of this article for non-commercial purposes provided that: the original authorship is properly and fully attributed; the Journal and Oxford University Press are attributed as the original place of publication with the correct citation details given; if an article is subsequently reproduced or disseminated not in its entirety but only in part or as a derivative work this must be clearly indicated. For commercial re-use, please contact journals.permissions@oxfordjournals.org

The human cumulus–oocyte complex gene-expression profile

Said Assou 1 , 2 , 3 , Tal Anahory 4 , Véronique Pantesco 2 , Tanguy Le Carrour 1 , Franck Pellestor 4 , 5 , Bernard Klein 1 , 2 , 3 , Lionel Reyftmann 6 , Hervé Dechaud 6 , John De Vos 1 , 2 , 3 , 7 and Samir Hamamah 1 , 2 , 3 , 4 , 8

1 CHU Montpellier, Institut de Recherche en BiothÉrapie, Hôpital Saint-Eloi, Montpellier, France; 2 INSERM, U 475 3 Université Montpellier1, UFR de médecine, Montpellier, France; 4 CHU Montpellier, Service de Biologie de la Reproduction B, Hôpital Arnaud de Villeneuve, Montpellier, France; 5 Institut de Génétique Humaine, CNRS UPR1142, Montpellier, France; and 6 CHU Montpellier, Service de Gynécologie-Obstétrique B, Hôpital Arnaud de Villeneuve, Montpellier, France

7 To whom correspondence should be addressed at: Research Institute for Biotherapy, HÔpital Saint-Eloi, 80 rue Augustin Fliche, 34295 Montpellier Cedex 5, France. E-mail: devos{at}montp.inserm.fr

8 To whom correspondence should be addressed at: CHU Montpellier, Service de Biologie de la Reproduction B, HÔpital Arnaud de Villeneuve, 371, av. du Doyen Gaston Giraud, 34295 Montpellier Cedex 5. E-mail: s-hamamah{at}chu-montpellier.fr

BACKGROUND: The understanding of the mechanisms regulating human oocyte maturation is still rudimentary. We have identified transcripts differentially expressed between immature and mature oocytes and cumulus cells. METHODS: Using oligonucleotide microarrays, genome-wide gene expression was studied in pooled immature and mature oocytes or cumulus cells from patients who underwent IVF. RESULTS: In addition to known genes, such as DAZL, BMP15 or GDF9, oocytes up-regulated 1514 genes. We show that PTTG3 and AURKC are respectively the securin and the Aurora kinase preferentially expressed during oocyte meiosis. Strikingly, oocytes overexpressed previously unreported growth factors such as TNFSF13/APRIL, FGF9, FGF14 and IL4 and transcription factors including OTX2, SOX15 and SOX30. Conversely, cumulus cells, in addition to known genes such as LHCGR or BMPR2, overexpressed cell-to-cell signalling genes including TNFSF11/RANKL, numerous complement components, semaphorins (SEMA3A, SEMA6A and SEMA6D) and CD genes such as CD200. We also identified 52 genes progressively increasing during oocyte maturation, including CDC25A and SOCS7. CONCLUSION: The identification of genes that were up- and down-regulated during oocyte maturation greatly improves our understanding of oocyte biology and will provide new markers that signal viable and competent oocytes. Furthermore, genes found expressed in cumulus cells are potential markers of granulosa cell tumours.

Key words: cumulus/germinal cells/microarray/oocytes


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