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Hum. Reprod. Advance Access originally published online on February 24, 2006
Human Reproduction 2006 21(7):1795-1797; doi:10.1093/humrep/del040
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© The Author 2006. Published by Oxford University Press on behalf of the European Society of Human Reproduction and Embryology. All rights reserved. For Permissions, please email: journals.permissions@oxfordjournals.org

research-article

A human tetraploid pachytene spermatocyte as the possible origin of diploid sperm: a case report

M. Codina-Pascual 1 , J. Navarro, J. Egozcue and J. Benet 1

Unitat de Biologia Cel·lular i Genètica Mèdica, Facultat de Medicina, Universitat Autònoma de Barcelona, Bellaterra, Spain

1 To whom correspondence should be addressed at: Departament de Biologia Cel·lular, Fisiologia i Immunologia, Facultat Medicina, Universitat Autònoma de Barcelona, Bellaterra 08193, Spain. E-mail: montserrat.codina{at}uab.es, jordi.benet{at}uab.es

Diploid spermatozoa represent 0.2–0.3% of all spermatozoa in the normal population and cause 8.3% of diandric triploids. Errors in meiosis I and II are the most common mechanisms by which diploid spermatozoa are produced. Endoreduplication before meiosis has been suggested as a possible origin for tetraploid meiocytes, which might, in turn, produce diploid sperm. Synaptonemal complex (SC) spreads of a fertile man were immunolabelled (SCP3, MLH1 and CENP) and hybridized with subtelomere-specific multiplex fluorescent in situ hybridization (stM-FISH) assay for SCs identification. The unexpected finding of a tetraploid pachytene cell and the identification of all of its SCs demonstrate that synapsis and crossover events can occur in human tetraploid cells. Moreover, it indicates that diploid sperm may also originate from mitotic errors (endoreduplication) occurring before meiosis.

Key words: crossing over/diploid sperm/stM-FISH/synaptonemal complex/tetraploid pachytene


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