Hum. Reprod. Advance Access originally published online on May 9, 2006
Human Reproduction 2006 21(8):2061-2064; doi:10.1093/humrep/del134
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Intra-individual variation in sperm chromatin structure assay parameters in men from infertile couples: clinical implications
1 Fertility Centre, Malmö University Hospital, Lund University, Malmö, Sweden 2 Andrology Laboratory, Riga Stradins University, Riga, Latvia 3 Fertility Clinic, Viborg Hospital (Skive), Skive, Denmark and 4 Section of Toxicology and Biomedical Sciences, BIOTEC-MED, ENEA CR Casaccia, Rome, Italy
5 To whom correspondence should be addressed at: Fertility Centre, Malmö University Hospital, Lund University, SE 205 02 Malmö, Sweden. E-mail: juris.erenpreiss{at}med.lu.se
BACKGROUND: Sperm DNA integrity is an important factor in the prognosis of male fertility. In this study, we investigated intra-individual variation of sperm chromatin structure assay (SCSA) parameters in infertility patients undergoing assisted reproductive techniques (ARTs). METHODS: Retrospective study of 282 consecutive patients referred for ART [intrauterine insemination (IUI), IVF or ICSI] with repeated (between 2 and 5) SCSA measurements. RESULTS: Mean coefficient of variation (CV) of DNA Fragmentation Index (DFI) for repeated SCSA measurements was 29%. A high proportion [37%; 95% confidence interval (CI): 27%, 49%] of patients with DFI >30% in the first test had DFI <30% in the second test. Also, a considerable proportion (27%; 95% CI : 16%, 40%) of patients with 21–30% DFI values in the first test had DFI >30% in the second test. CONCLUSIONS: Intra-individual variability in DFI is significant, therefore repeated SCSA measurements are recommended. The biological mechanisms behind these variations remain to be elucidated.
Key words: flow cytometry/infertility/SCSA/sperm chromatin/sperm DNA integrity
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