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Hum. Reprod. Advance Access originally published online on May 23, 2006
Human Reproduction 2006 21(9):2346-2352; doi:10.1093/humrep/del163
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© The Author 2006. Published by Oxford University Press on behalf of the European Society of Human Reproduction and Embryology. All rights reserved. For Permissions, please email: journals.permissions@oxfordjournals.org

Association of spermatogenic failure with decreased CDC25A expression in infertile men

Yu Sheng Cheng1,2, Pao Lin Kuo3, Yen Ni Teng4, Ting Yi Kuo1, Chia Ling Chung3, Ying Hung Lin5, Rui Wen Liao3, Johnny Shinn Nan Lin1 and Yung Ming Lin1,6

1 Department of Urology 2 Institute of Clinical Medicine 3 Department of Obstetrics & Gynecology, National Cheng Kung University, College of Medicine 4 Department of Early Childhood Education and Nursery, Chia Nan University of Pharmacy and Science and 5 Institute of Basic Medical Science, National Cheng Kung University, College of Medicine, Tainan, Taiwan

6 To whom correspondence should be addressed at: Department of Urology, National Cheng Kung University, College of Medicine, 138, Sheng Li Road, Tainan, Taiwan. E-mail: linym{at}mail.ncku.edu.tw

BACKGROUND: DAZ gene family is crucial for human spermatogenesis that requires the precise co-ordination of cell cycle events. CDC25A is recognized as the downstream substrate of DAZ gene family and is thought to function on the M-phase regulation of cell cycles. We investigated the expression profiles of CDC25A in the testes of infertile men and evaluated the relationship between CDC25A levels and testicular phenotype, clinical hormonal parameters and sperm retrieval results. METHODS: The protein and mRNA transcript levels of CDC25A in the testes of 40 azoospermic men were determined by immunohistochemistry and quantitative real-time-PCR. CDC25A in human spermatozoa was investigated by western blotting and immunofluorescence staining. RESULTS: The CDC25A protein was expressed mainly in spermatocyte, spermatid and spermatozoa. CDC25A transcript levels were significantly decreased (P = 0.0009) in patients with spermatogenic failure, especially in men with meiotic arrest and Sertoli cell-only syndrome. Significantly higher CDC25A transcript levels were detected in patients with successful sperm retrieval than in patients with failed sperm retrieval (P = 0.005). CONCLUSIONS: Decreased CDC25A is associated with spermatogenic failure and failed sperm retrieval in infertile men. Further studies are necessary to explore the functional roles of CDC25A in human spermatozoa.

Key words: CDC25A/male infertility/testis


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