Hum. Reprod. Advance Access originally published online on September 13, 2006
Human Reproduction 2007 22(1):197-200; doi:10.1093/humrep/del351
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
First recorded pregnancy and normal birth after ICSI using electrophoretically isolated spermatozoa
1 Reproductive Science Group, Discipline of Biological Sciences, University of Newcastle, Callaghan, NSW 2 Sydney IVF and Department of Obstetrics and Gynaecology, University of Sydney, Sydney and 3 ARC Centre of Excellence in Biotechnology and Development, University of Newcastle, Callaghan, NSW, Australia
4 To whom correspondence should be addressed at: Discipline of Biological Sciences, School of Environmental and Life Sciences, Faculty of Science and IT, University of Newcastle, Callaghan, NSW 2308, Australia. E-mail: jaitken{at}mail.newcastle.edu.au
BACKGROUND: DNA damage in the male germ line is associated with poor fertilization and cleavage rates, impaired embryo quality and early pregnancy loss. Given these associations, embryologists are keen to develop techniques that will allow the selection of viable spermatozoa exhibiting low levels of DNA damage for assisted conception purposes. METHODS: In this article, we describe a novel electrophoretic approach for the rapid isolation of cells possessing little DNA damage. The limits of the method were examined using cryostored and snap-frozen semen samples as well as testicular biopsy material. In addition, clinical utility was demonstrated in a case study involving treatment of a patient exhibiting persistently high levels of DNA damage in his spermatozoa. RESULTS: From a range of difficult starting materials (biopsies, cryostored semen and snap-frozen sperm suspensions), the electrophoretic system rapidly isolated populations of motile, viable, morphologically normal spermatozoa exhibiting high levels of DNA integrity. Clinical application in a couple suffering from long-term infertility associated with extensive DNA damage in the male germ line led to the first human pregnancy following such electrophoretic sperm isolation. CONCLUSIONS: The electrophoretic procedure holds promise as a convenient method for the rapid preparation of high-quality spermatozoa for assisted conception purposes.
Key words: assisted conception/DNA damage/electrophoretic method/human spermatozoa/sperm isolation
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  S.D. Fleming, R.S. Ilad, A-M.G. Griffin, Y. Wu, K.J. Ong, H.C. Smith, and R.J. Aitken Prospective controlled trial of an electrophoretic method of sperm preparation for assisted reproduction: comparison with density gradient centrifugation Hum. Reprod., September 1, 2008; (2008) den330v1. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. M. Said, A. Agarwal, M. Zborowski, S. Grunewald, H.-J. Glander, and U. Paasch Utility of Magnetic Cell Separation as a Molecular Sperm Preparation Technique J Androl, March 1, 2008; 29(2): 134 - 142. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Bungum, M. Spano, P. Humaidan, P. Eleuteri, M. Rescia, and A. Giwercman Sperm chromatin structure assay parameters measured after density gradient centrifugation are not predictive for the outcome of ART Hum. Reprod., January 1, 2008; 23(1): 4 - 10. [Abstract] [Full Text] [PDF]
|
|