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Hum. Reprod. Advance Access originally published online on August 12, 2006
Human Reproduction 2007 22(1):36-44; doi:10.1093/humrep/del328
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© The Author 2006. Published by Oxford University Press on behalf of the European Society of Human Reproduction and Embryology. All rights reserved. For Permissions, please email: journals.permissions@oxfordjournals.org

Seminal plasma and prostaglandin E2 up-regulate fibroblast growth factor 2 expression in endometrial adenocarcinoma cells via E-series prostanoid-2 receptor-mediated transactivation of the epidermal growth factor receptor and extracellular signal-regulated kinase pathway

S. Battersby1, K.J. Sales1, A.R. Williams2, R.A. Anderson3, S. Gardner1 and H.N. Jabbour1,4

1 Medical Research Council Human Reproductive Science Unit and 2 Department of Pathology and 3 Reproductive and Developmental Sciences, Centre for Reproductive Biology, The Queen’s Medical Research Institute, Little France Crescent, Edinburgh, UK

4 To whom correspondence should be addressed at: Medical Research Council Human Reproductive Science Unit, Centre for Reproductive Biology, The Queen’s Medical Research Institute, 47 Little France Crescent, Edinburgh, EH16 4TJ, UK. E-mail: h.jabbour{at}hrsu.mrc.ac.uk

BACKGROUND: Prostaglandin E2 (PGE2) has been shown to modulate angiogenesis and tumour progression via the E-series prostanoid-2 (EP2) receptor. Endometrial adenocarcinomas may be exposed to endogenous PGE2 and exogenous PGE2, present at high concentration in seminal plasma. METHODS: This study investigated fibroblast growth factor 2 (FGF2) mRNA expression and cell signalling in response to seminal plasma or PGE2, using an endometrial adenocarcinoma (Ishikawa) cell line stably expressing the EP2 receptor (EP2 sense cells) and endometrial adenocarcinoma explants. RESULTS: Seminal plasma and PGE2 induced a significant up-regulation of FGF2 expression in EP2 sense but not parental untransfected Ishikawa (wild-type) cells (P < 0.05). These effects were inhibited by co-treatment with EP2 receptor antagonist or inhibitors of protein kinase A, c-Src, epidermal growth factor receptor (EGFR) kinase or extracellular signal-regulated kinase (ERK) signalling. The treatment of EP2 sense cells with seminal plasma induced cAMP accumulation and phosphorylation of c-Src, EGFR kinase and ERK via the EP2 receptor. Finally, seminal plasma and PGE2 significantly increased FGF2 mRNA expression in endometrial adenocarcinoma tissue explants via the EP2 receptor (P < 0.05). CONCLUSIONS: Seminal plasma and PGE2 can similarly activate FGF2 expression and EP2 receptor signalling in endometrial adenocarcinoma cells. These data highlight the potential for seminal plasma exposure to facilitate tumorigenesis–angiogenesis in endometrial adenocarcinomas in vivo.

Key words: endometrial carcinoma/E-series prostanoid receptor 2/fibroblast growth factor 2/seminal plasma


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K. J. Sales, S. C. Boddy, A. R. W. Williams, R. A. Anderson, and H. N. Jabbour
F-Prostanoid Receptor Regulation of Fibroblast Growth Factor 2 Signaling in Endometrial Adenocarcinoma Cells
Endocrinology, August 1, 2007; 148(8): 3635 - 3644.
[Abstract] [Full Text] [PDF]


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