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Hum. Reprod. Advance Access originally published online on September 14, 2007
Human Reproduction 2007 22(11):2903-2911; doi:10.1093/humrep/dem265
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© The Author 2007. Published by Oxford University Press on behalf of the European Society of Human Reproduction and Embryology. All rights reserved. For Permissions, please email: journals.permissions@oxfordjournals.org

Co-expression of two perivascular cell markers isolates mesenchymal stem-like cells from human endometrium

K.E. Schwab and C.E. Gargett1

Centre for Women's Health Research, Monash Institute of Medical Research, Monash University Department of Obstetrics and Gynaecology, Monash Medical Centre, 246 Clayton Road, Victoria 3168, Australia

1 Correspondence address. Tel: +61-3-9594-5392; Fax: +61-3-9594-6389; E-mail: caroline.gargett{at}med.monash.edu.au

BACKGROUND: Human endometrium has immense regenerative capacity, growing ~5 mm in 7 days every month. We have previously identified a small population of colony-forming endometrial stromal cells which we hypothesize are mesenchymal stem cells (MSC). The aim of this study was to determine if the co-expression of two perivascular cell markers, CD146 and platelet-derived growth factor-receptor β (PDGF-Rβ), will prospectively isolate endometrial stromal cells which exhibit MSC properties, and determine their location in human endometrium.

METHODS: Single cell suspensions of human endometrial stromal cells were fluorescence activated cell sorting (FACS) sorted into CD146+PDGF-Rβ+ and CD146–PDGF-Rβ– populations and analysed for colony-forming ability, in vitro differentiation and expression of typical MSC markers. Full thickness human endometrial sections were co-stained for CD146 and PDGF-Rβ.

RESULTS: FACS stromal CD146+PDGF-Rβ+ stromal cells (1.5% of sorted population) were enriched for colony-forming cells compared with CD146–PDGF-Rβ– cells (7.7 ± 1.7 versus 0.7 ± 0.2% P <0.0001), and also underwent differentiation into adipogenic, osteogenic, myogenic and chondrogenic lineages. They expressed MSC phenotypic surface markers and were located near blood vessels.

CONCLUSION: This study shows that human endometrium contains a small population of MSC-like cells that may be responsible for its cyclical growth, and may provide a readily available source of MSC for tissue engineering applications.

Key words: human endometrium/mesenchymal stem cells/cell surface markers/in vitro differentiation/clonal assays

Submitted on May 21, 2007; resubmitted on July 12, 2007; accepted on July 24, 2007.


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