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Hum. Reprod. Advance Access originally published online on October 19, 2007
Human Reproduction 2007 22(12):3069-3077; doi:10.1093/humrep/dem336
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© The Author 2007. Published by Oxford University Press on behalf of the European Society of Human Reproduction and Embryology. All rights reserved. For Permissions, please email: journals.permissions@oxfordjournals.org

Gene expression in human cumulus cells: one approach to oocyte competence

P. Feuerstein1,2, V. Cadoret1,3, R. Dalbies-Tran1, F. Guerif1,2,3, R. Bidault3 and D. Royere1,2,3,4

1 INRA, CNRS, Université de Tours, Haras Nationaux, UMR Physiologie de la Reproduction et des Comportements, 37380 Nouzilly, France 2 Université François Rabelais de Tours, UFR Médecine, 37041 Tours, France 3 CHRU de Tours, Laboratoire de Biologie de la Reproduction, 37044 Tours, France

4 Correspondence address. E-mail: royere{at}med.univ-tours.fr

BACKGROUND: Dialogue between the oocyte and cumulus cells is essential for oocyte maturation. A prospective laboratory research project was designed to evaluate transcription of specific genes in cumulus cells harvested before intracytoplasmic sperm injection from pre-ovulatory follicles, according to individual oocyte nuclear maturity and developmental competence. Genes were chosen because their expression was induced by the LH peak [Steroidogenic Acute Regulatory protein (STAR), Cyclooxygenase 2 (COX2 or PTGS2), Amphiregulin (AREG)] or because they were involved in oocyte lipidic metabolism [Stearoyl-Coenzyme A Desaturase 1 and 5 (SCD1 and SCD5)] or in gap-junctions [Connexin 43 (CX43 or GJA1)].

METHODS: mRNA levels in cumulus cells were assessed by real-time PCR.

RESULTS: Expression levels of all genes investigated, except Cx43, were increased after resumption of meiosis. Nuclear maturation was thus associated with increased expression of STAR, COX2, AREG, SCD1 and SCD5 by cumulus cells. When considering only cumulus associated with metaphase II oocytes, gene expression was independent of morphological status at Day 2. In contrast, transcript levels were lower and distributed over a narrower range in cumulus enclosing oocytes achieving blastocyst development at Day 5/6 than in cumulus enclosing oocytes unable to develop beyond the embryo stage.

CONCLUSION: Further developmental potential from embryo to blastocyst stage was associated with lower expression in a narrow range for these genes.

Key words: oocyte maturation/human cumulus cells/gene expression/developmental competence/resumption of meiosis

Submitted on July 11, 2007; resubmitted on August 17, 2007; accepted on September 12, 2007.


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