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Hum. Reprod. Advance Access originally published online on November 10, 2006
Human Reproduction 2007 22(2):401-406; doi:10.1093/humrep/del408
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© The Author 2006. Published by Oxford University Press on behalf of the European Society of Human Reproduction and Embryology. All rights reserved. For Permissions, please email: journals.permissions@oxfordjournals.org

Gonadotrophin stimulation of non-luteinized granulosa cells increases steroid production and the expression of enzymes involved in estrogen and progesterone synthesis

M. Lindeberg1,3, K. Carlström1, O. Ritvos2 and O. Hovatta1

1 Department of Clinical Science Investigation and Technology, Division of Obstetrics and Gynecology, Karolinska Institutet, Karolinska University Hospital Huddinge, Stockholm, Sweden 2 Programme for Developmental and Reproductive Biology, Biomedicum Helsinki and Department of Bacteriology and Immunology, Haartman Institute, 00014 University of Helsinki, Helsinki, Finland

3 To whom correspondence should be addressed at: Department of Obstetrics and Gynecology, K57, Karolinska Institute, Karolinska University Hospital Huddinge, S-141 86 Stockholm, Sweden. E-mail: maria.lindeberg{at}ki.se

BACKGROUND: In regular IVF treatment, mature oocytes are collected with their luteinized granulosa cells (GCs). When in vitro maturation (IVM) of the oocytes is performed, non-luteinized GCs can be collected. We have investigated how these cells respond to gonadotrophin stimulation in culture. METHODS: GCs were collected from patients undergoing IVM treatment and compared with GCs from IVF patients. The cells were stimulated with FSH and/or hCG. After 48 h, culture media were collected for hormone analysis, and RNA was isolated for gene expression analysis. RESULTS: In IVM GCs, hCG and FSH alone and in combination induced significantly increased progesterone production, and FSH alone and in combination with hCG increased estrogen production. We also studied the gene expression of P-450aromatase and P-450scc and the receptors for FSH and LH. In non-luteinized GCs, the expression levels of P-450aromatase increased with all treatments, and P-450scc expression increased with the combined FSH and hCG treatment. LHR expression increased with FSH treatment, but the FSH receptor expression did not change with different treatments. CONCLUSIONS: Non-luteinized GCs behaved differently from luteinized GCs in culture. The data help understand the final stages of maturation of human oocytes and follicles.

Key words: in vitro maturation of human oocytes/immature granulosa cells/gonadotrophin receptors/aromatase/culture


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