Hum. Reprod. Advance Access originally published online on March 8, 2007
Human Reproduction 2007 22(6):1526-1531; doi:10.1093/humrep/dem029
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High bone morphogenetic protein-15 level in follicular fluid is associated with high quality oocyte and subsequent embryonic development
Department of Reproductive Endocrinology, Women's Hospital, School of Medicine, Zhejiang University, Hangzhou, Zhejiang, People's Republic of China
1 To whom correspondence should be addressed at: Department of Reproductive Endocrinology, Women's Hospital, School of Medicine, Zhejiang University, 2 Xue Shi Road, Hangzhou, Zhejiang 310006, People's Republic of China. E-mail: huanghefg{at}hotmail.com
BACKGROUND: Bone morphogenetic protein-15 (BMP-15) has been shown to influence oocyte maturation and quality. However, no relationship has been established between BMP-15 and oocyte quality/embryonic development in humans. The aim of this study is to investigate BMP-15 level in human follicular fluid (FF) and its possible role in determining oocyte quality and developmental potential.
METHODS: A total of 79 occytes and their corresponding FF from 79 women undergoing ICSI were examined. Individual oocytes were inseminated and subsequently assessed on the basis of their fertilization, cleavage and preimplantation development. BMP-15, FSH, estradiol (E2) and progesterone levels of FF were also analysed via the techniques of western blot or radioimmunoassay.
RESULTS: Higher FF BMP-15 levels were observed in the fertilized and cleaved groups versus the unfertilized and uncleaved groups, respectively (P < 0.05). The best (Grade I) embryo morphology was associated with higher FF BMP-15 levels than Grade II or III embryos (P < 0.01). A significant positive correlation was found between BMP-15 and E2 levels in the same follicle.
CONCLUSION: The present study demonstrates that the BMP-15 level in FF appears to be a potential factor in predicting oocyte quality and subsequent embryo development, and is correlated with E2 level, which may additionally be a valuable predictor of oocyte fertilization.
Key words: bone morphogenetic protein-15/embryonic development/follicular fluid/IVF/oocyte
* The authors equally contributed to this work
Submitted on August 30, 2006; resubmitted on November 22, 2006; resubmitted on December 8, 2006; accepted on December 15, 2006.
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