Hum. Reprod. Advance Access originally published online on November 6, 2007
Human Reproduction 2008 23(1):4-10; doi:10.1093/humrep/dem353
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Sperm chromatin structure assay parameters measured after density gradient centrifugation are not predictive for the outcome of ART
1 Centre of Reproductive Medicine, Malmö University Hospital, 205 02 Malmö, Sweden 2 Section of Toxicology and Biomedical Sciences, BAS-BIOTEC-MED, ENEA Casaccia Research Center, Rome, Italy 3 The Fertility Clinic, Viborg Hospital (Skive), 7800 Skive, Denmark
4 Correspondence address. E-mail: mona.bungum{at}med.lu.se
BACKGROUND: The sperm chromatin structure assay (SCSA) parameter DNA fragmentation index (DFI) has been shown to predict in vivo and in vitro fertility. So far most SCSA studies have been based on SCSA analysis performed on neat semen. The aim of this study is to assess whether SCSA analysis of sperm prepared by density gradient centrifugation (DGC) could add more information in regard to the prediction of treatment outcome.
METHODS: The study included 510 assisted reproductive technique (ART) cycles. SCSA was performed in neat semen and post DGC. SCSA results were expressed in terms of DFI and high DNA stainability (HDS) cell fractions. The outcome parameter was clinical pregnancy (CP).
RESULTS: Scatter-plot diagrams demonstrated that for DGC samples, no DFI cut-off values could be set for in vivo or in vitro fertility. In intrauterine insemination, IVF and ICSI groups the mean difference (95% CI) in DFI post DGC between those who achieved CP and those who did not was 0.2% (–1.7 to 2.0%), 0.4% (–1.9 to 2.8%) and 1.3% (–3.1 to 5.9%), respectively, none of these being statistically significant. The corresponding differences for HDS were 0.1% (–1.3 to 1.5%), 0.1% (–0.7 to 0.9%) and 0.6% (–1.6 to 2.7%), respectively (all P-values >0.6).
CONCLUSIONS: SCSA performed in semen prepared by DGC cannot predict the outcome of ART.
Key words: sperm chromatin structure assay/density gradient centrifugation/DNA fragmentation index/high DNA stainability/assisted reproduction
Submitted on August 21, 2007; resubmitted on October 1, 2007; accepted on October 8, 2007.
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