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Hum. Reprod. Advance Access originally published online on July 8, 2008
Human Reproduction 2008 23(10):2185-2193; doi:10.1093/humrep/den137
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© The Author 2008. Published by Oxford University Press on behalf of the European Society of Human Reproduction and Embryology. All rights reserved. For Permissions, please email: journals.permissions@oxfordjournals.org
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Similar biological characteristics of human embryonic stem cell lines with normal and abnormal karyotypes

Xiaofang Sun1, Xiaolin Long, Yifei Yin, Yonghua Jiang, Xinjie Chen, Weiqiang Liu, Wenhong Zhang, Hongzi Du, Shaoying Li, Yuhong Zheng, Shu Kong, Qianying Pang, Yu Shi, Yulin Huang, Shengchan Huang, Baoping Liao, Guohong Xiao and Weihua Wang

Institute of Gynecology and Obstetrics, The Third Affiliated Hospital of Guangzhou Medical College, Duobao Road, Guangzhou, People's Republic of China

1 Correspondence address. Tel: +86-20-81292202; Fax: +86-20-81292013; E-mail: xiaofangsun{at}hotmail.com

BACKGROUND: Human embryonic stem cell (hESC) lines derived from poor quality embryos usually have either normal or abnormal karyotypes. However, it is still unclear whether their biological characteristics are similar.

METHODS: Seven new hESC lines were established using discarded embryos. Five cell lines had normal karyotype, one was with an unbalanced Robertsonian translocation and one had a triploid karyotype. Their biological characteristics, short tandem repeat loci, HLA typing, differentiation capability and imprinted gene, DNA methylation and X chromosome inactivation status were compared between different cell lines.

RESULTS: All seven hESC lines had similar biological characteristics regardless of karyotype (five normal and two abnormal), such as expression of stage-specific embryonic antigen (SSEA)-4, tumor-rejection antigen (TRA)-1-81 and TRA-1-60 proteins, transcription factor octamer binding protein 4 mRNA, no detectable expression of SSEA-1 protein and high levels of alkaline phosphatase activity. All cell lines were able to undergo differentiation. Imprinted gene expression and DNA methylation were also similar among these cell lines. Non-random X chromosome inactivation patterns were found in XX cell lines.

CONCLUSIONS: The present results suggest that hESC lines with abnormal karyotype are also useful experimental materials for cell therapy, developmental biology and genetic research.

Key words: human embryonic stem cell lines/characterization/karyotype/methylation/X-inactivation

Submitted on January 11, 2008; resubmitted on March 16, 2008; accepted on April 1, 2008.


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