Hum. Reprod. Advance Access originally published online on July 3, 2008
Human Reproduction 2008 23(10):2266-2272; doi:10.1093/humrep/den244
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Two successful pregnancies following autotransplantation of frozen/thawed ovarian tissue
1 Laboratory of Reproductive Biology, Section 5712, University Hospital of Copenhagen, Blegdamsvej 9, Rigshospitalet, DK-2100 Copenhagen, Denmark 2 The Fertility Clinic, University Hospital of Copenhagen, Copenhagen, Denmark 3 Gynaecological Department at The Juliane Marie Centre for Women, Children and Reproduction, University Hospital of Copenhagen, Copenhagen, Denmark 4 Department of Gynaecology and Obstetrics, Aarhus University Hospital, Aarhus, Denmark 5 Reproductive Laboratory, Institute of Anatomy, University of Aarhus, Aarhus, Denmark
6 Correspondence address. Tel: +45-35455822; Fax: +45-35455824; E-mail: yding{at}rh.dk
BACKGROUND: Cryopreservation of the ovarian cortex with subsequent autotransplantation has, on an experimental basis, been performed to preserve fertility in women being treated for a malignant disease. The present study reports ovarian activity and pregnancies following autotransplantation of frozen/thawed ovarian tissue.
METHODS: One complete ovary was cryopreserved from each of six patients who were 26–35 years old prior to treatment. Tissue from three of the patients was transported 4–5 h on ice prior to cryopreservation. After a period of 17–32 months, orthotopic autotransplantation was performed replacing 20–60% of the tissue. Two patients received additional heterotopic transplants.
RESULTS: In all cases, the tissue restored menstrual cyclicity 14–20 weeks following transplantation. Four of the six women conceived following assisted reproduction: two women (who had the tissue transported 4–5 h prior to cryopreservation) each, based on the orthotopic transplanted tissue, delivered one healthy child (February 2007 and January 2008); one woman miscarriaged in gestational Week 7; and the other had a positive hCG test but no clinical pregnancy. The remaining two women did not become pregnant.
CONCLUSIONS: Two additional healthy children have been born as a result of the ovarian cryopreservation procedure. In both cases, the ovarian tissue was transported 4–5 h prior to freezing demonstrating that hospitals may offer cryopreservation without having the necessary expertise locally.
Key words: ovary/cryopreservation/autotransplantation/pregnancies/assisted reproduction
Submitted on February 26, 2008; resubmitted on May 28, 2008; accepted on June 2, 2008.
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