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Hum. Reprod. Advance Access originally published online on January 7, 2008
Human Reproduction 2008 23(3):581-588; doi:10.1093/humrep/dem345
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© The Author 2008. Published by Oxford University Press on behalf of the European Society of Human Reproduction and Embryology. All rights reserved. For Permissions, please email: journals.permissions@oxfordjournals.org

Sperm fluorescence in situ hybridization study of meiotic segregation and an interchromosomal effect in carriers of t(11;18)

Miluse Vozdova1, Eva Oracova1,2, Vera Horinova3 and Jiri Rubes1,2,4

1 Department of Genetics and Reproduction, Veterinary Research Institute, Hudcova 70, 621 00 Brno, Czech Republic 2 Center for Reproductive Medicine REPROMEDA, Vinicni 235, 615 00 Brno, Czech Republic 3 Sanatorium Helios SIVF, Stefanikova 12, 602 00 Brno, Czech Republic

4Correspondence address. E-mail: rubes{at}vri.cz

BACKGROUND: alanced translocations are associated with infertility, spontaneous abortions and birth defects.

METHODS: We report the analysis, by multicolour fluorescence in situ hybridization (FISH), of meiotic segregation and aneuploidy of chromosomes X, Y, 7, 8 and 21 in sperm from three men who are carriers of two different translocations involving chromosomes 11 and 18. A control group comprised ten young, healthy normospermic men.

RESULTS: the higher prevalence of alternate segregation followed by adjacent 1, adjacent 2 and 3:1, and other segregants was observed in all three patients. Two carriers of the same translocation differed only in the frequency of adjacent 2 segregation (P < 0.01). The carrier of the other translocation showed significantly higher frequency of alternate (P < 0.01) and less adjacent 1 and 3:1 segregation products (P < 0.01). An increased frequency of XY (P < 0.01), YY (P < 0.05) and diploid (P < 0.01) sperm was also detected in the group of translocation carriers compared with the control group. This difference was caused by elevated frequencies of disomy and diploidy in two of our carriers.

CONCLUSIONS: the incidence of chromosomally unbalanced or aneuploid gametes varies in the individual translocation carriers even if the same chromosomes are included in the translocation. FISH analysis provides information useful for genetic counseling and assisted reproduction.

Key words: meiotic segregation/reciprocal translocation/interchromosomal effect

Submitted on June 29, 2007; resubmitted on August 27, 2007; accepted on September 26, 2007.


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