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Hum. Reprod. Advance Access originally published online on December 22, 2007
Human Reproduction 2008 23(3):651-661; doi:10.1093/humrep/dem380
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© The Author 2007. Published by Oxford University Press on behalf of the European Society of Human Reproduction and Embryology. All rights reserved. For Permissions, please email: journals.permissions@oxfordjournals.org

Proteomic analysis of human omental adipose tissue in the polycystic ovary syndrome using two-dimensional difference gel electrophoresis and mass spectrometry

Marta Cortón1, José I. Botella-Carretero2,3, Juan A. López4, Emilio Camafeita4, José L. San Millán2,3, Héctor F. Escobar-Morreale2,3 and Belén Peral1,5

1 Instituto de Investigaciones Biomédicas, Consejo Superior de Investigaciones Científicas and Universidad Autónoma de Madrid, E-28029 Madrid, Spain 2 Department of Endocrinology, Hospital Universitario Ramón y Cajal and Universidad de Alcalá, E-28034 Madrid, Spain 3 Department of Molecular Genetics, Hospital Universitario Ramón y Cajal and Universidad de Alcalá, E-28034 Madrid, Spain 4 Unidad de Proteómica, Centro Nacional de Investigaciones Cardiovasculares, E-28029 Madrid, Spain

5Correspondence address. E-mail: bperal{at}iib.uam.es

BACKGROUND: Our aim was to study the protein expression profiles of omental adipose tissue biopsies obtained from morbidly obese women with or without polycystic ovary syndrome (PCOS) at the time of bariatric surgery to evaluate the possible involvement of visceral adiposity in the development of PCOS.

METHODS: Ten PCOS patients and nine control samples were included. We used two-dimensional difference gel electrophoresis (2D-DIGE) followed by in-gel digestion, and mass spectrometry (MS) of selected protein spots.

RESULTS: The 2D-DIGE technology allowed the analysis of ~1840 protein spots in the comparative study of control and patient proteomes, revealing 15 statistically significant spot changes (>2-fold, P < 0.05). Unambiguous protein identification was achieved for 9 of these 15 spots by MS. This preliminary study revealed differences in expression of proteins that may be involved in lipid and glucose metabolism, oxidative stress processes and adipocyte differentiation; they include proapolipoprotein Apo-A1, annexin V, glutathione S-transferase M3 (GSTM3), triosephosphate isomerase, peroxiredoxin 2 isoform a, actin and adipocyte plasma membrane-associated protein. The most relevant finding was an increase of GSTM3 in the omental fat of PCOS patients confirming previous studies conducted by our group.

CONCLUSIONS: Proteomic analysis of omental fat reveals differential expression of several proteins in PCOS patients and non-hyperandrogenic women presenting with morbid obesity. The application of this novel methodology adds further evidence to support the role of visceral adiposity in the pathogenesis of PCOS.

Key words: ovary/polycystic/proteomics/hyperandrogenism/visceral adipose tissue

Submitted on June 25, 2007; resubmitted on September 24, 2007; accepted on November 2, 2007.


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